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Article

Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages

  • Authors:
    • Jihua Chen
    • Takuhiro Uto
    • Shunsuke Tanigawa
    • Tomeo Yamada-Kato
    • Makoto Fujii
    • De-Xing Hou
  • View Affiliations / Copyright

    Affiliations: Course of Biological Science and Technology, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan
  • Pages: 33-40
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/etm_00000006
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Abstract

6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a bioactive ingredient of wasabi [Wasabia japonica (Miq.) Matsumura], which is a popular pungent spice of Japan. To evaluate the anti-inflammatory function and underlying genes targeted by 6-MSITC, gene expression profiling through DNA microarray was performed in mouse macrophages. Among 22,050 oligonucleotides, the expression levels of 406 genes were increased by ≥3-fold in lipopolysaccharide (LPS)-activated RAW264 cells, 238 gene signals of which were attenuated by 6-MSITC (≥2-fold). Expression levels of 717 genes were decreased by ≥3-fold in LPS-activated cells, of which 336 gene signals were restored by 6-MSITC (≥2-fold). Utilizing group analysis, 206 genes affected by 6-MSITC with a ≥2-fold change were classified into 35 categories relating to biological processes (81), molecular functions (108) and signaling pathways (17). The genes were further categorized as ‘defense, inflammatory response, cytokine activities and receptor activities’ and some were confirmed by real-time polymerase chain reaction. Ingenuity pathway analysis further revealed that wasabi 6-MSITC regulated the relevant networks of chemokines, interleukins and interferons to exert its anti-inflammatory function.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Uto T, Tanigawa S, Yamada-Kato T, Fujii M and Hou D: Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages . Exp Ther Med 1: 33-40, 2010.
APA
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., & Hou, D. (2010). Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages . Experimental and Therapeutic Medicine, 1, 33-40. https://doi.org/10.3892/etm_00000006
MLA
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., Hou, D."Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages ". Experimental and Therapeutic Medicine 1.1 (2010): 33-40.
Chicago
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., Hou, D."Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 33-40. https://doi.org/10.3892/etm_00000006
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Uto T, Tanigawa S, Yamada-Kato T, Fujii M and Hou D: Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages . Exp Ther Med 1: 33-40, 2010.
APA
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., & Hou, D. (2010). Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages . Experimental and Therapeutic Medicine, 1, 33-40. https://doi.org/10.3892/etm_00000006
MLA
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., Hou, D."Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages ". Experimental and Therapeutic Medicine 1.1 (2010): 33-40.
Chicago
Chen, J., Uto, T., Tanigawa, S., Yamada-Kato, T., Fujii, M., Hou, D."Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 33-40. https://doi.org/10.3892/etm_00000006
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