Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-April 2010 Volume 1 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-April 2010 Volume 1 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro

  • Authors:
    • Youko Itoh
    • Yoshiaki Yamada
    • Yoshiaki Kazaoka
    • Tsuneo Ishiguchi
    • Nobuaki Honda
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Aichi Medical University School of Medicine, Aichi 480-1195, Japan
  • Pages: 319-323
    |
    Published online on: March 1, 2010
       https://doi.org/10.3892/etm_00000049
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Although the combined effects of hyperthermia and chemotherapy for cancer therapy have been theoretically established, combination therapy with hyperthermia is not widely used clinically, since the application of hyperthermia is complex and maintaining a tumor temperature of 43°C or above is very difficult. Thus, in the present study, the combined effects of chemotherapy and mild hyperthermia, which is easier to apply than hyperthermia, were examined using a human bladder cancer cell line, T24. T24 cells were incubated at 37-47°C for 0-5 h, and the survival rate of cells was measured using the MTT method. Cisplatin and adriamycin, which are indicated for human bladder cancer, were used as anti-tumor drugs. In the combination experiment, T24 cells were treated with cisplatin (20 and 200 µg/ml) or adriamycin (4 and 40 µg/ml) at 41°C for 0-5 h and at 37°C for 7-12 h. After a total incubation of 12 h, cell survival was measured by the MTT method. i) T24 cells, like cells in general, survived mild hyperthermia at 41°C, but were killed at over 43°C. ii) Although a low concentration (20 µg/ml) of cisplatin or of mild hyperthermia at 41°C did not affect the survival rate of T24 cells, this combination therapy decreased the survival rate of T24 cells with increasing incubation times at 41°C. The anti-tumor effect of the combination of 20 µg/ml cisplatin and 41°C for 5 h was the same as that of 10 times the concentration (200 µg/ml) of cisplatin. iii) Adriamycin showed the same effects as cisplatin. The anti-tumor effect of the combination of 4 µg/ml adriamycin and 41°C for 5 h was identical to the anti-tumor effect of a 10-fold higher concentration (40 µg/ml) of adriamycin. Based on these results, combination with mild hyperthermia may enable a reduction in the dose of anti-tumor drugs. It was suggested that the side effects of chemotherapy could be reduced clinically by combining the drugs with mild hyperthermia. Mild hyperthermia might be a useful and practical heating method which could lead to the increased clinical applications of hyperthermia. We previously reported the preliminary results of M-VAC chemotherapy (methotrexate, vinblastine, doxorubicin and cisplatin) combined with mild hyperthermia, a new therapeutic strategy for advanced or metastatic transitional cell carcinoma of the urothelium. This basic research strongly supports the clinical beneficial results of chemotherapy combined with mild hyperthermia.
View Figures

Figure 1

Figure 2

Figure 3

View References

1. 

Sternberg CN, Yagoda A, Scher HI, et al: Preliminary results of M-VAC (methotrexate, vinblastine, doxorubicin and cisplatin) for transitional cell carcinoma of the urothelium. J Urol. 133:403–407. 1985.PubMed/NCBI

2. 

Masse HM, Hansen SW, Robert JT, et al: Gemcitabine and cisplatin versus methotrexate, vinblastine, doxorubicin and cisplatin in advanced or metastatic bladder cancer; results of a large, randomized, multinational, multicancer, phase III study. J Clin Oncol. 18:3068–3077. 2000.

3. 

Saxman SB, Propert KJ, Einborn LH, et al: Long-term follow-up of a phase III intergroup study of cisplatin alone or in combination with methotrexate, vinblastine and doxorubicin in patients with metastatic urothelial carcinoma; a cooperative group study. J Clin Oncol. 15:2564–2569. 1997.PubMed/NCBI

4. 

Sternberg CN, Yagoda A, Scher HI, et al: Methotrexate, vinblastine, doxorubicin and cisplatin for advanced transitional cell carcinoma of the urothelium. Efficacy and patterns of response and relapse. Cancer. 64:2448–2458. 1989. View Article : Google Scholar : PubMed/NCBI

5. 

Mizuno S: Biological and medical grounds of combination with hyperthermia and radiotherapy. Hyperthermia Manual (in Japanese). Matsuda T: Magupuros Publication Co.; pp. 1–5. 1991

6. 

Mitsuhashi N: Positioning in combination therapy and future subjects. Hyperthermia – Guide Book of Hyperthermia for Cancer Therapy (in Japanese). Japanese Society for Thermal Medicine, Mainichi-kenkousaron Publication Co.; pp. 6–7. 2008

7. 

Itoh Y, Kazaoka Y, Nitta M, et al: Combining anti-tumor drugs with mild hyperthermia increases the cytotoxic effects of drugs on human leukemia cells in vitro. Mol Med Rep. 2:411–415. 2009.PubMed/NCBI

8. 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

9. 

Zwelling LA and Kohn KW: Mechanism of action of cis-dichlorodiammineplatinum (II). Cancer Treat Rep. 63:1439–1444. 1979.PubMed/NCBI

10. 

Di M: Adriamycin (NSC-123127): mode and mechanism of action. Cancer Chemother Rep. 6:91–106. 1975.

11. 

Nemoto T and Takahira H: Uptake of adriamycin into the cells and the interaction with DNA. Yakugakuzasshi (in Japanese). 93:1498–1508. 1973.

12. 

Kameda K, Kondo T, Tanabe K, et al: The role of intracellular Ca2+ in apoptosis induced by hyperthermia and its enhancement by verapamil in U937 cells. Int J Radiat Oncol. 49:1369–1379. 2001.PubMed/NCBI

13. 

Hall EJ and Roizin T: Biological effects of heat. Cancer Res. 44:S4708–S4713. 1984.

14. 

Shioura H, Hayashi S, Matsumoto H, et al: The effects of combined treatments with low hyperthermia and bleomycin on survival of murine L cells. Clin Cancer Res. 16:147–152. 1997.PubMed/NCBI

15. 

Kano E, Furukawa-Furuya M and Nitta K: Sensitivity of bleomycin-resistant variant cells enhanced by 40°C hyperthermia in vitro. Int J Hyperthermia. 4:5547–5553. 1988.PubMed/NCBI

16. 

Ono H, Ando S and Suzuki T: The drug uptake in the tumor with the mild-hyperthermia treatment in combination with the chemotherapy in vivo. Jpn J Hyperthermic Oncol. 22:23–33. 2006. View Article : Google Scholar

17. 

Huang Q, Hu JK and Lohr F: Heat-induced gene expression as a novel targeted cancer gene therapy strategy. Cancer Res. 60:3435–3439. 2000.PubMed/NCBI

18. 

Urano M, Kuroda M and Nishimura Y: For the clinical application of thermal chemotherapy given at mild temperatures. Int J Hyperthermia. 15:79–107. 1999. View Article : Google Scholar

19. 

Ahmed K, Hori T and Yu DA: Hyperthermia chemo-sensitization, chemical thermo-sensitization and apoptosis. Thermal Med. 24:1–12. 2008. View Article : Google Scholar

20. 

Itoh Y, Tazawa K, Wada K, et al: Induction of HSP 70 in lymphocytes by whole body far-infrared hyperthermia. Jpn J Hyperthermic Oncol. 21:209–220. 2005.

21. 

Itoh Y, Ogawa K and Tazawa K: Improvement of athletic performances by heat shock protein 70 induced with mild hyperthermia. Jpn J Clin Physiol. 38:13–21. 2008.

22. 

Itoh Y and Yamada Y: Enhancement of the anti-tumor effects and improvement of QOL by the combination with mild hyperthermia. In: 67th Annual Meeting of the Japanese Cancer Association (JCA 2008) – Proceedings; pp. 3612008

23. 

Yamada Y, Itoh Y and Honda Y: Increase of anti-tumor effect by combination therapy with mild hyperthermia and chemotherapy for bladder cancer. Thermal Med (in Japanese). 24:S652008.

24. 

Yamada Y, Itoh Y, Aoki S, et al: Preliminary results of M-VAC chemotherapy combined with mild hyperthermia, a new therapeutic strategy for advanced or metastatic transitional cell carcinoma of the urothelium. Cancer Chemother Pharmacol. 64:1079–1083. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Itoh Y, Yamada Y, Kazaoka Y, Ishiguchi T and Honda N: Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro . Exp Ther Med 1: 319-323, 2010.
APA
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., & Honda, N. (2010). Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro . Experimental and Therapeutic Medicine, 1, 319-323. https://doi.org/10.3892/etm_00000049
MLA
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., Honda, N."Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro ". Experimental and Therapeutic Medicine 1.2 (2010): 319-323.
Chicago
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., Honda, N."Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro ". Experimental and Therapeutic Medicine 1, no. 2 (2010): 319-323. https://doi.org/10.3892/etm_00000049
Copy and paste a formatted citation
x
Spandidos Publications style
Itoh Y, Yamada Y, Kazaoka Y, Ishiguchi T and Honda N: Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro . Exp Ther Med 1: 319-323, 2010.
APA
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., & Honda, N. (2010). Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro . Experimental and Therapeutic Medicine, 1, 319-323. https://doi.org/10.3892/etm_00000049
MLA
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., Honda, N."Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro ". Experimental and Therapeutic Medicine 1.2 (2010): 319-323.
Chicago
Itoh, Y., Yamada, Y., Kazaoka, Y., Ishiguchi, T., Honda, N."Combination of chemotherapy and mild hyperthermia enhances the anti-tumor effects of cisplatin and adriamycin in human bladder cancer T24 cells in vitro ". Experimental and Therapeutic Medicine 1, no. 2 (2010): 319-323. https://doi.org/10.3892/etm_00000049
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team