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Article

Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma

  • Authors:
    • Prasong Khaenam
    • Patcharee Jearanaikoon
    • Chawalit Pairojkul
    • Vajarabhongsa Bhudhisawasdi
    • Temduang Limpaiboon
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
  • Pages: 385-390
    |
    Published online on: March 1, 2010
       https://doi.org/10.3892/etm_00000060
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Abstract

The retinoblastoma interacting zinc finger (RIZ1) gene is adjacent to D1S228 where microsatellite instability has been associated with poor patient survival in liver fluke-associated cholangiocarcinoma (CCA). An understanding of the molecular mechanisms underlying the carcinogenesis and pathogenesis of CCA is necessary to improve patient survival. Therefore, we determined the genetic and epigenetic alterations of RIZ1 in 81 CCA samples and 69 matched non-tumor tissues. Methylation was found in 31 of 81 (38%) tumor samples and in 5 of 69 (7%) matched non-tumor tissues. Frameshift mutations (2 of 81) and loss of heterozygosity (LOH) (14 of 81) were not common. Statistical analysis found no significant correlation between RIZ1 alterations and clinicopathological features, but RIZPro704 LOH was associated with patient survival in the multivariate analysis. RIZ1 hypermethylation may be one of the crucial molecular events contributing to cholangiocarcinogenesis, and RIZPro704 LOH may adversely impact patient survival. The biological function of RIZ1 in CCA should be further investigated in order to verify its potential role in regulating this cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Khaenam P, Jearanaikoon P, Pairojkul C, Bhudhisawasdi V and Limpaiboon T: Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma . Exp Ther Med 1: 385-390, 2010.
APA
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., & Limpaiboon, T. (2010). Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma . Experimental and Therapeutic Medicine, 1, 385-390. https://doi.org/10.3892/etm_00000060
MLA
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., Limpaiboon, T."Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma ". Experimental and Therapeutic Medicine 1.2 (2010): 385-390.
Chicago
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., Limpaiboon, T."Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma ". Experimental and Therapeutic Medicine 1, no. 2 (2010): 385-390. https://doi.org/10.3892/etm_00000060
Copy and paste a formatted citation
x
Spandidos Publications style
Khaenam P, Jearanaikoon P, Pairojkul C, Bhudhisawasdi V and Limpaiboon T: Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma . Exp Ther Med 1: 385-390, 2010.
APA
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., & Limpaiboon, T. (2010). Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma . Experimental and Therapeutic Medicine, 1, 385-390. https://doi.org/10.3892/etm_00000060
MLA
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., Limpaiboon, T."Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma ". Experimental and Therapeutic Medicine 1.2 (2010): 385-390.
Chicago
Khaenam, P., Jearanaikoon, P., Pairojkul, C., Bhudhisawasdi, V., Limpaiboon, T."Genetic and epigenetic alterations of RIZ1 and the correlation to clinicopathological parameters in liver fluke-related cholangiocarcinoma ". Experimental and Therapeutic Medicine 1, no. 2 (2010): 385-390. https://doi.org/10.3892/etm_00000060
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