Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-June 2010 Volume 1 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-June 2010 Volume 1 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Involvement of calpain in colorectal adenocarcinomas (Review)

  • Authors:
    • Ponniah Selvakumar
    • Rajendra K. Sharma
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, and Cancer Research Unit, Saskatoon Cancer Center, University of Saskatchewan, Saskatchewan S7N 4H4, Canada
  • Pages: 413-417
    |
    Published online on: May 1, 2010
       https://doi.org/10.3892/etm_00000064
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Calpains represent a well-conserved family of Ca2+-dependent proteolytic enzymes. Recently, the importance of calpain in the metastatic process has received a great deal of attention. Various reports have suggested that m-calpain contributes to the pathogenesis of various cancers, including colorectal cancer. The activity and protein expression of m-calpain was significantly higher in colorectal adenocarcinoma than in normal colonic mucosa as revealed by Western blotting and immunohistochemical analysis. In addition, the decreased expression of calpain inhibitors (calpastatin and high molecular weight calmodulin-binding protein) was correlated with the increased activity and expression of calpain in colorectal adenocarcinoma. This has implications with regard to the design of chemotherapeutic drugs as well as for the monitoring of colorectal cancer in the early stages of the metastatic process. In this review, we summarize some of the recent findings from our laboratory regarding calpain and its inhibitors in human colon cancer.
View Figures

Figure 1

Figure 2

View References

1. 

Sorimachi H, Ishiura S and Suzuki K: Structure and physiological function of calpains. Biochem J. 328:721–732. 1997.

2. 

Goll DE, Thompson VF, Li H, Wei W and Cong J: The calpain system. Physiol Rev. 83:731–801. 2002. View Article : Google Scholar

3. 

Ono Y, Sorimachi H and Suzuki K: Structure and physiology of calpain, an enigmatic protease. Biochem Biophys Res Commun. 245:289–294. 1998. View Article : Google Scholar : PubMed/NCBI

4. 

Maki M, Ma H, Takano E, Adachi Y, Lee WJ, Hatanaka M and Murachi T: Calpastatins: biochemical and molecular biological studies. Biomed Biochim Acta. 50:509–516. 1991.PubMed/NCBI

5. 

Carragher NO: Calpain inhibition: a therapeutic strategy targeting multiple disease states. Curr Pharm Des. 12:615–638. 2006. View Article : Google Scholar : PubMed/NCBI

6. 

Sharma RK: Purification and characterization of novel calmodulin-binding protein from cardiac muscle. J Biol Chem. 265:1152–1157. 1990.PubMed/NCBI

7. 

Kakkar R, Raju RV, Mellgren RL, Radhi J and Sharma RK: Cardiac high molecular weight calmodulin binding protein contains calpastatin activity. Biochemistry. 36:11550–11555. 1997. View Article : Google Scholar

8. 

Carragher NO and Frame MC: Calpain: a role in cell transformation and migration. Int J Biochem Cell Biol. 34:1539–1543. 2002. View Article : Google Scholar : PubMed/NCBI

9. 

Huttenlocher A, Palecek SP, Lu Q, Zhang W, Mellgren RL, Lauffenburger DA, Ginsberg MH and Horwitz AF: Regulation of cell migration by the calcium-dependent protease calpain. J Biol Chem. 272:32719–32722. 1997. View Article : Google Scholar : PubMed/NCBI

10. 

Patel YM and Lane MD: Mitotic clonal expansion during pre-adipocyte differentiation: calpain-mediated turnover of p27. J Biol Chem. 275:17653–17660. 2000. View Article : Google Scholar : PubMed/NCBI

11. 

Lakshmikuttyamma A, Selvakumar P, Kakkar R, Kanthan R, Wang R and Sharma RK: Activation of calcineurin expression in ischemia-reperfused rat heart and in human ischemic myocardium. J Cell Biochem. 90:987–997. 2003. View Article : Google Scholar : PubMed/NCBI

12. 

Lakshmikuttyamma A, Selvakumar P, Sharma AR, Anderson DH and Sharma RK: In vitro proteolytic degradation of bovine brain calcineurin by m-calpain. Neurochem Res. 29:1913–1921. 2004. View Article : Google Scholar : PubMed/NCBI

13. 

Lakshmikuttyamma A, Selvakumar P, Charavaryamath C, Singh B, Tuchek J and Sharma RK: Expression of calcineurin and its interacting proteins in epileptic fowl. J Neurochem. 96:366–373. 2006. View Article : Google Scholar : PubMed/NCBI

14. 

Lakshmikuttyamma A, Selvakumar P, Tuchek J and Sharma RK: Myristoyltransferase and calcineurin: novel molecular therapeutic target for epilepsy. Prog Neurobiol. 84:77–84. 2008. View Article : Google Scholar : PubMed/NCBI

15. 

Selvakumar P, Lakshmikuttyamma A, Charavaryamath C, Singh B, Tuchek J and Sharma RK: Expression of myristoyltransferase and its interacting proteins in epilepsy. Biochem Biophys Res Commun. 335:1132–1139. 2005. View Article : Google Scholar : PubMed/NCBI

16. 

Kishimoto A, Kajikawa N, Shiota M and Nishizuka Y: Preoteolytic activation of calcium-activated, phospholipid-dependent protein kinase by calcium-dependent neutral protease. J Biol Chem. 258:1156–1164. 1983.

17. 

Oda A, Drucker BJ, Ariyoshi H, Smith M and Salzman E: pp60src is an andogenous substrate for calpain in human blood platelets. J Biol Chem. 268:12603–12608. 1993.

18. 

Frangioni JV, Oda A, Smith M, Salzman EW and Neel BG: Calpain-catalyzed cleavage and subcellular relocation of protein phosphotyrosine phosphatase 1B PTB-1B in human platelets. EMBO J. 12:4843–4856. 1993.

19. 

Braun C, Engel M, Seifert M, Theisinger B, Seitz G, Zang KD and Welter C: Expression of calpain I messenger RNA in human renal cell carcinoma: correlation with lymph node metastasis and histological type. Int J Cancer. 84:6–9. 1999. View Article : Google Scholar : PubMed/NCBI

20. 

Mamoune A, Luo JH, Lauffenburger DA and Wells A: Calpain-2 is a target for limiting prostate cancer invasion. Cancer Res. 63:4632–4640. 2003.PubMed/NCBI

21. 

Yoshikawa Y, Mukai H, Hino F, Asada K and Kato I: Isolation of two novel genes, down regulated in gastric cancer. Jpn J Cancer Res. 91:459–463. 2000. View Article : Google Scholar : PubMed/NCBI

22. 

Liu K, Li L and Cohen SN: Antisense RNA-mediated deficiency of the calpain protease, nCL-4, in NH3T3cells is associated with neoplastic transformation and tumorigenesis. J Biol Chem. 275:31093–31098. 2000. View Article : Google Scholar : PubMed/NCBI

23. 

Witkowski JM, Zmuda-Trzebiatowska E, Swiercz JM, Cichorek M, Ciepluch H, Lewandowski K, Bryl E and Hellmann A: Modulation of the activity of calcium-activated neutral proteases calpains in chronic lymphocytic leukemia B-CLL cells. Blood. 100:1802–1809. 2002. View Article : Google Scholar : PubMed/NCBI

24. 

Carillo S, Pariat M, Steff AM, Roux P, Etienne-Julan M, Lorca T and Piechaczyk M: Differential sensitivity of FOS and JUN family members to calpains. Oncogene. 9:1679–1689. 1994.PubMed/NCBI

25. 

Kubbutat MH and Vousden KH: Proteolytic cleavage of human p53 by calpain: a potential regulator of protein stability. Mol Cell Biol. 17:460–468. 1997.PubMed/NCBI

26. 

Carragher NO, Fincham VJ, Riley D and Frame MC: Cleavage of focal adhesion kinase by different proteases during SRC-regulated transformation and apoptosis. Distinct roles for calpain and caspases. J Biol Chem. 276:4270–4275. 2001. View Article : Google Scholar : PubMed/NCBI

27. 

Johnson D, Frame MC and Wyke JA: Expression of the v-Src oncoprotein in fibroblasts disrupts normal regulation of the CDK inhibitor p27 and inhibits quiescence. Oncogene. 16:2017–2028. 1998. View Article : Google Scholar : PubMed/NCBI

28. 

Kellie S: Cellular transformation, tyrosine kinase oncogenes and the cellular adhesion plaque. Bioessays. 8:25–30. 1988. View Article : Google Scholar : PubMed/NCBI

29. 

Carragher NO, Westhoff MA, Riley D, Potter DA, Dutt P, Elce JS, Greer PA and Frame MC: v-Src-induced modulation of the calpain-calpastatin proteolytic system regulates transformation. Mol Cell Biol. 22:257–269. 2002. View Article : Google Scholar : PubMed/NCBI

30. 

Bolen JB, Veillette A, Schwartz AM, DeSeau V and Rosen N: Activation of pp60c-src protein kinase activity in human colon carcinoma. Proc Natl Acad Sci USA. 84:2251–2255. 1987.PubMed/NCBI

31. 

Ottenhoff-Kalff AE, Rijksen G, van Beurden EA, Hennipman A, Michels AA and Staal GE: Characterization of protein tyrosine kinases from human breast cancer: involvement of the c-src oncogene product. Cancer Res. 52:4773–4778. 1992.PubMed/NCBI

32. 

Lakshmikuttyamma A, Selvakumar P, Kanthan R, Kanthan SC and Sharma RK: Overexpression of m-calpain in human colorectal adenocarcinomas. Cancer Epidemiol Biomarkers Prev. 13:1604–1609. 2004.PubMed/NCBI

33. 

Selvakumar P, Smith-Windsor E, Bonham K and Sharma RK: N-myristoyltransferase 2 expression in human colon cancer: cross-talk between the calpain and caspase system. FEBS Lett. 580:2021–2026. 2006. View Article : Google Scholar : PubMed/NCBI

34. 

Raju RV, Kakkar R, Datla RS, Radhi J and Sharma RK: Myristoyl-coA: protein N-myristoyltransferase from bovine cardiac muscle: molecular cloning, kinetic analysis and in vitro proteolytic cleavage by m-calpain. Exp Cell Res. 241:23–35. 1998. View Article : Google Scholar : PubMed/NCBI

35. 

Levine AJ: p53, the cellular gatekeeper for growth and division. Cell. 88:323–331. 1997. View Article : Google Scholar : PubMed/NCBI

36. 

Giaccia AJ and Kastan MB: The complexity of p53 modulation: emerging patterns from divergent signals. Genes Dev. 12:2973–2983. 1998. View Article : Google Scholar : PubMed/NCBI

37. 

Gonen H, Shkedy D, Barnoy S, Kosower NS and Ciechanover A: On the involvement of calpains in the degradation of the tumor suppressor protein p53. FEBS Lett. 406:17–22. 1997. View Article : Google Scholar : PubMed/NCBI

38. 

Toyota H, Yanase N, Yoshimoto T, Moriyama M, Sudo T and Mizuguchi J: Calpain-induced Bax-cleavage product is a more potent inducer of apoptotic cell death than wild-type Bax. Cancer Lett. 189:221–230. 2003. View Article : Google Scholar : PubMed/NCBI

39. 

Blomgren K, Zhu C, Wang X, Karlsson JO, Leverin AL, Bahr BA, Mallard C and Hagberg H: Synergistic activation of caspase-3 by m-calpain after neonatal hypoxia-ischemia: a mechanism of ‘pathological apoptosis’? J Biol Chem. 276:10191–10198. 2001.PubMed/NCBI

40. 

Ruiz-Vela A, Gonzalez de Buitrago G and Martinez AC: Implication of calpain in caspase activation during B cell clonal deletion. EMBO J. 18:4988–4998. 1999. View Article : Google Scholar : PubMed/NCBI

41. 

Chen T, Yang I, Irby R, Shain KH, Wang HG, Quackenbush J, Coppola D, Cheng JQ and Yeatman TJ: Regulation of caspase expression and apoptosis by adenomatous polyposis coli. Cancer Res. 63:4368–4374. 2003.PubMed/NCBI

42. 

Zhu DM and Uckun FM: Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin's lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 6:2456–2463. 2000.PubMed/NCBI

43. 

Raju RV, Moyana TN and Sharma RK: N-myristoyltransferase overexpression in human colorectal adenocarcinomas. Exp Cell Res. 235:145–154. 1997. View Article : Google Scholar : PubMed/NCBI

44. 

Moyana TN and Xiang J: Expression of tumor-associated polymorphic epithelial mucin and carcinoembryonic antigen in gastrointestinal carcinoid tumors. Cancer. 75:2836–2843. 1995. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Selvakumar P and Sharma RK: Involvement of calpain in colorectal adenocarcinomas (Review) . Exp Ther Med 1: 413-417, 2010.
APA
Selvakumar, P., & Sharma, R.K. (2010). Involvement of calpain in colorectal adenocarcinomas (Review) . Experimental and Therapeutic Medicine, 1, 413-417. https://doi.org/10.3892/etm_00000064
MLA
Selvakumar, P., Sharma, R. K."Involvement of calpain in colorectal adenocarcinomas (Review) ". Experimental and Therapeutic Medicine 1.3 (2010): 413-417.
Chicago
Selvakumar, P., Sharma, R. K."Involvement of calpain in colorectal adenocarcinomas (Review) ". Experimental and Therapeutic Medicine 1, no. 3 (2010): 413-417. https://doi.org/10.3892/etm_00000064
Copy and paste a formatted citation
x
Spandidos Publications style
Selvakumar P and Sharma RK: Involvement of calpain in colorectal adenocarcinomas (Review) . Exp Ther Med 1: 413-417, 2010.
APA
Selvakumar, P., & Sharma, R.K. (2010). Involvement of calpain in colorectal adenocarcinomas (Review) . Experimental and Therapeutic Medicine, 1, 413-417. https://doi.org/10.3892/etm_00000064
MLA
Selvakumar, P., Sharma, R. K."Involvement of calpain in colorectal adenocarcinomas (Review) ". Experimental and Therapeutic Medicine 1.3 (2010): 413-417.
Chicago
Selvakumar, P., Sharma, R. K."Involvement of calpain in colorectal adenocarcinomas (Review) ". Experimental and Therapeutic Medicine 1, no. 3 (2010): 413-417. https://doi.org/10.3892/etm_00000064
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team