Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro

Hasegawa,Tomokazu; Chosa,Naoyuki; Asakawa,Takeyoshi; Yoshimura,Yoshitaka; Asakawa,Asami; Ishisaki,Akira; Tanaka,Mitsuro

doi:10.3892/etm_00000074

May-June 2010 Volume 1 Issue 3

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May-June 2010 Volume 1 Issue 3

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Article

Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro

Authors:
- Tomokazu Hasegawa
- Naoyuki Chosa
- Takeyoshi Asakawa
- Yoshitaka Yoshimura
- Asami Asakawa
- Akira Ishisaki
- Mitsuro Tanaka
View Affiliations / Copyright

Affiliations: Department of Pediatric Dentistry, School of Dentistry, Iwate Medical University, Morioka 020-8505, Japan. hasegawa@iwate-med.ac.jp
Pages: 477-480
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Published online on: May 1, 2010

https://doi.org/10.3892/etm_00000074
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Abstract

Although dental pulp (DP) cells are indispensable for repair after injury, and blood supply is crucial for tissue healing and regeneration, the potential of DP cells derived from deciduous teeth to express endothelial cell (EC)-specific markers has yet to be determined. Therefore, this study investigated mRNA expression of the EC-specific markers vascular endothelial-cadherin (VE-cadherin), vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 in DP cells derived from human deciduous teeth and treated with 10 ng/ml fibroblast growth factor (FGF)-2 in vitro. Quantitative PCR was used to determine the mRNA expression levels of the EC-specific markers, and showed that the FGF-2-cultured DP cells exhibited mRNA expression of VE-cadherin and VEGFR2, and marked CD31 mRNA expression. Western blot analysis showed that CD31 protein was induced in the DP cells following 3 weeks of treatment with FGF-2. DP cells derived from deciduous teeth inducibly expressed EC-specific markers, and thus have the potential to differentiate into cells of the vascular lineage.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro

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