Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro

  • Authors:
    • Tomokazu Hasegawa
    • Naoyuki Chosa
    • Takeyoshi Asakawa
    • Yoshitaka Yoshimura
    • Asami Asakawa
    • Akira Ishisaki
    • Mitsuro Tanaka
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/etm_00000074
  • Pages: 477-480
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Abstract

Although dental pulp (DP) cells are indispensable for repair after injury, and blood supply is crucial for tissue healing and regeneration, the potential of DP cells derived from deciduous teeth to express endothelial cell (EC)-specific markers has yet to be determined. Therefore, this study investigated mRNA expression of the EC-specific markers vascular endothelial-cadherin (VE-cadherin), vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 in DP cells derived from human deciduous teeth and treated with 10 ng/ml fibroblast growth factor (FGF)-2 in vitro. Quantitative PCR was used to determine the mRNA expression levels of the EC-specific markers, and showed that the FGF-2-cultured DP cells exhibited mRNA expression of VE-cadherin and VEGFR2, and marked CD31 mRNA expression. Western blot analysis showed that CD31 protein was induced in the DP cells following 3 weeks of treatment with FGF-2. DP cells derived from deciduous teeth inducibly expressed EC-specific markers, and thus have the potential to differentiate into cells of the vascular lineage.
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May-June 2010
Volume 1 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hasegawa T, Chosa N, Asakawa T, Yoshimura Y, Asakawa A, Ishisaki A and Tanaka M: Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro . Exp Ther Med 1: 477-480, 2010
APA
Hasegawa, T., Chosa, N., Asakawa, T., Yoshimura, Y., Asakawa, A., Ishisaki, A., & Tanaka, M. (2010). Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro . Experimental and Therapeutic Medicine, 1, 477-480. https://doi.org/10.3892/etm_00000074
MLA
Hasegawa, T., Chosa, N., Asakawa, T., Yoshimura, Y., Asakawa, A., Ishisaki, A., Tanaka, M."Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro ". Experimental and Therapeutic Medicine 1.3 (2010): 477-480.
Chicago
Hasegawa, T., Chosa, N., Asakawa, T., Yoshimura, Y., Asakawa, A., Ishisaki, A., Tanaka, M."Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro ". Experimental and Therapeutic Medicine 1, no. 3 (2010): 477-480. https://doi.org/10.3892/etm_00000074