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Article

Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins

  • Authors:
    • Bishnu Acharya
    • Shuji Terao
    • Toru Suzuki
    • Michio Naoe
    • Katsuyuki Hamada
    • Hiroyuki Mizuguchi
    • Akinobu Gotoh
  • View Affiliations / Copyright

    Affiliations: Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo 663-8501, Japan
  • Pages: 537-540
    |
    Published online on: May 1, 2010
       https://doi.org/10.3892/etm_00000085
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Abstract

The transduction efficacy of adenovirus serotype 5 (Ad5) vector in human renal carcinoma cells is generally low due to the down-regulated expression of Coxsackie and adenovirus receptor (CAR) in target cells. By contrast, the infectivity of adenovirus serotype 35 vectors depends on the binding rate to CD46 receptor, independent of CAR. In this study, we examined whether an adenovirus vector containing chimeric type 5 and type 35 fiber proteins (Ad5/F35) increases transduction efficiency compared to Ad5 vector in human renal carcinoma cells in vitro. The expression of CAR was much lower in the human renal carcinoma cells than in control HEK293 cells. By contrast, the expression of CD46 was similar and perhaps at a higher level in the human renal carcinoma cells than in the HEK293 cells. The transduction efficacy of Ad5/F35 vector was dramatically higher compared to that of Ad5 in human renal carcinoma cells, and was correlated to the expression of CD46. Thus, Ad5/35 vector may be useful for the development of novel gene therapy approaches to renal cell carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Acharya B, Terao S, Suzuki T, Naoe M, Hamada K, Mizuguchi H and Gotoh A: Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins . Exp Ther Med 1: 537-540, 2010.
APA
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., & Gotoh, A. (2010). Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins . Experimental and Therapeutic Medicine, 1, 537-540. https://doi.org/10.3892/etm_00000085
MLA
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., Gotoh, A."Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins ". Experimental and Therapeutic Medicine 1.3 (2010): 537-540.
Chicago
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., Gotoh, A."Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins ". Experimental and Therapeutic Medicine 1, no. 3 (2010): 537-540. https://doi.org/10.3892/etm_00000085
Copy and paste a formatted citation
x
Spandidos Publications style
Acharya B, Terao S, Suzuki T, Naoe M, Hamada K, Mizuguchi H and Gotoh A: Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins . Exp Ther Med 1: 537-540, 2010.
APA
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., & Gotoh, A. (2010). Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins . Experimental and Therapeutic Medicine, 1, 537-540. https://doi.org/10.3892/etm_00000085
MLA
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., Gotoh, A."Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins ". Experimental and Therapeutic Medicine 1.3 (2010): 537-540.
Chicago
Acharya, B., Terao, S., Suzuki, T., Naoe, M., Hamada, K., Mizuguchi, H., Gotoh, A."Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins ". Experimental and Therapeutic Medicine 1, no. 3 (2010): 537-540. https://doi.org/10.3892/etm_00000085
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