Re-assessment of chronic radio-induced tissue damage in a rat hindlimb model

  • Authors:
    • Bérengère Phulpin
    • Gilles Dolivet
    • Pierre-Yves Marie
    • Sylvain Poussier
    • Patrice Gallet
    • Agnès Leroux
    • Pierre Graff
    • Frederique Groubach
    • Pierre Bravetti
    • Jean-Louis Merlin
    • Nguyen Tran
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/etm_00000087
  • Pages: 553-560
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Abstract

Radiotherapy is successfully used to treat neoplastic lesions, but may adversely affect normal tissues within the irradiated volume. However, additional clinical and paraclinical data are required for a comprehensive understanding of the pathogenesis of this damage. We assessed a rat model using clinical records and medical imaging to gain a better understanding of irradiation-induced tissue damage. The hindlimbs of the rats in this model were irradiated with a single dose of 30 or 50 Gy. Sequential analysis was based on observation records of stage and planar scintigraphy. Additional radiography, radiohistology and histology studies were performed to detect histological alterations. All animals developed acute and late effects, with an increased severity after a dose of 50 Gy. The bone uptake of 99mTc-HDP was significantly decreased in a dose- and time-dependent manner. Histologically, significant tissue damage was observed. After the 50 Gy irradiation, the animals developed lesions characteristic of osteoradionecrosis (ORN). Radiographic and histological studies provided evidence of lytic bone lesions. Our rat model developed tissue damage characteristic of radiation injury after a single 30 Gy irradiation and tissue degeneration similar to that which occurs during human ORN after a 50 Gy irradiation. The development of this animal model is an essential step in exploring the pathogenesis of irradiation-induced tissue damage, and may be used to test the efficacy of new treatments.
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July-August 2010
Volume 1 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Phulpin B, Dolivet G, Marie P, Poussier S, Gallet P, Leroux A, Graff P, Groubach F, Bravetti P, Merlin J, Merlin J, et al: Re-assessment of chronic radio-induced tissue damage in a rat hindlimb model . Exp Ther Med 1: 553-560, 2010
APA
Phulpin, B., Dolivet, G., Marie, P., Poussier, S., Gallet, P., Leroux, A. ... Tran, N. (2010). Re-assessment of chronic radio-induced tissue damage in a rat hindlimb model . Experimental and Therapeutic Medicine, 1, 553-560. https://doi.org/10.3892/etm_00000087
MLA
Phulpin, B., Dolivet, G., Marie, P., Poussier, S., Gallet, P., Leroux, A., Graff, P., Groubach, F., Bravetti, P., Merlin, J., Tran, N."Re-assessment of chronic radio-induced tissue damage in a rat hindlimb model ". Experimental and Therapeutic Medicine 1.4 (2010): 553-560.
Chicago
Phulpin, B., Dolivet, G., Marie, P., Poussier, S., Gallet, P., Leroux, A., Graff, P., Groubach, F., Bravetti, P., Merlin, J., Tran, N."Re-assessment of chronic radio-induced tissue damage in a rat hindlimb model ". Experimental and Therapeutic Medicine 1, no. 4 (2010): 553-560. https://doi.org/10.3892/etm_00000087