Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-August 2010 Volume 1 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-August 2010 Volume 1 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Case Report

Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study

  • Authors:
    • Shigeto Matsushita
    • Ko-Ichi Tada
    • Ko-Ichi Kawahara
    • Kazuhiro Kawai
    • Teruto Hashiguchi
    • Ikuro Maruyama
    • Takuro Kanekura
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Field of Sensory Organology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan
  • Pages: 569-574
    |
    Published online on: July 1, 2010
       https://doi.org/10.3892/etm_00000089
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Malignant melanoma (MM) is an aggressive chemoresistant skin cancer characterized by rapid metastasis and a poor prognosis. Therefore, the development of innovative effective therapies is critical. MK615 is an extract from the Japanese apricot Prunus mume Sieb. Et Zucc (Ume). At a neutral pH, it contains natural chemical substances such as triterpenoids that exert anti-neoplastic effects in several types of cancers. We found that in patients with advanced MM, MK615 dramatically suppressed the cutaneous in-transit metastasis of the disease. Pre- and post-treatment comparison of tumors showed that the apoptotic index was significantly increased by MK615. In vitro studies, MTT assay, flow cytometric cell cycle analysis and immunofluorescence microscopy revealed that MK615 inhibited the growth of SK-MEL28 cells in a dose-dependent manner, increased the proportion of cells in sub-G1 phase and induced apoptosis. We further examined the expression of the receptor for advanced glycation end products (RAGE). RAGE is a multi-ligand receptor that binds to a novel cytokine, high mobility group box protein 1 (HMGB1), as well as advanced glycation end products. There is evidence that RAGE/HMGB1 interactions enhance cell invasion in MM. Here, we present Western blotting and immunofluorescence microscopy data indicating that MK615 inhibited the expression of RAGE in SK-MEL28 cells, and suppressed the release of HMGB1 by SK-MEL28 cells. Our findings suggest that MK615 may be a valuable tool for treating MM and other malignant tumors.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1. 

Houghton AN and Polsky D: Focus on melanoma. Cancer Cell. 2:275–278. 2002. View Article : Google Scholar

2. 

Soengas MS and Lowe SW: Apoptosis and melanoma chemo-resistance. Oncogene. 22:3138–3151. 2003. View Article : Google Scholar : PubMed/NCBI

3. 

Adachi M, Suzuki Y, Mizuta T, Osawa T, Suzuki K, Shiojima K, Arai Y, Masuda K, Uchiyama M and Oyamada T: The Japanese apricot ‘Prunus mume Sieb. Et Zucc’ (Ume) is a rich natural source of novel anti-cancer substance. Int J Food Prop. 10:375–384. 2007.

4. 

Xu R, Frazio GC and Matsuda PT: On the origins of triterpenoid skeletal diversity. Phytochemistry. 65:261–291. 2004. View Article : Google Scholar : PubMed/NCBI

5. 

Gutterman JU, La HT, Yang P, Haridas V, Gaikwad A and Marcus S: Effects of the tumor inhibitory triterpenoid avicin G on cell integrity, cytokinesis, and protein ubiquitination in fission yeast. Proc Natl Acad Sci USA. 102:12771–12776. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Ramachandran C, Rabi T, Fonseca HB, Melnick SJ and Escalon EA: Novel plant triterpenoid drug amooranin overcomes multidrug resistance in human leukemia and colon carcinoma cell lines. Int J Cancer. 105:784–789. 2003. View Article : Google Scholar : PubMed/NCBI

7. 

Nakagawa A, Sawada T, Okada T, Ohsawa T, Adachi M and Kubota K: New antineoplastic agent, MK615, from Ume (a variety of) Japanese apricot inhibits growth of breast cancer cells in vitro. Breast J. 13:44–49. 2007. View Article : Google Scholar : PubMed/NCBI

8. 

Okada T, Sawada T, Osawa T, Adachi M and Kubota K: A novel anti-cancer substance, MK615, from ume, a variety of Japanese apricot, inhibits growth of hepatocellular carcinoma cells by suppressing Aurora A kinase activity. Hepatogastroenterology. 54:1770–1774. 2007.

9. 

Mori S, Sawada T, Okada T, Ohsawa T, Adachi M and Keiichi K: New anti-proliferative agent, MK615, from Japanese apricot ‘Prunus mume’ induces striking autophagy in colon cancer cells in vitro. World J Gastroenterol. 13:6512–6517. 2007.PubMed/NCBI

10. 

Okada T, Sawada T, Osawa T, Adachi M and Kubota K: MK615 inhibits pancreatic cancer cell growth by dual inhibition of Aurora A and B kinases. World J Gastroenterol. 14:1378–1382. 2008. View Article : Google Scholar : PubMed/NCBI

11. 

Ansari B, Coates PJ, Greenstein BD and Hall PA: In situ end-labeling detects DNA strand breaks in apoptosis and other physiological and pathological states. J Pathol. 170:1–8. 1993. View Article : Google Scholar : PubMed/NCBI

12. 

Biswas KK, Sarker KP, Abeyama K, Kawahara K, Iino S, Otsubo Y, Saigo K, Izumi H, Hashiguchi T, Yamakuchi M, Yamaji K, Endo R, Suzuki K, Imaizumi H and Haruyama I: Membrane cholesterol but not putative receptors mediates anandamide-induced hepatocyte apoptosis. Hepatology. 38:1167–1177. 2003. View Article : Google Scholar : PubMed/NCBI

13. 

Kawahara K, Setoyama K, Kikuchi K, Biswas KK, Kamimura R, Iwata M, Ito T, Morimoto Y, Hashiguchi T, Takao S and Maruyama I: HMGB1 release in co-cultures of porcine endothelial and human T cells. Xenotransplantation. 14:636–641. 2007. View Article : Google Scholar : PubMed/NCBI

14. 

Morimoto Y, Kawahara KI, Tancharoen S, Kikuchi K, Matuyama T, Hashiguchi T, Izumi Y and Maruyama I: Tumor necrosis factor-alpha stimulates gingival epithelial cells to release high mobility-group box 1. J Periodontal Res. 43:76–83. 2008. View Article : Google Scholar : PubMed/NCBI

15. 

Zar JH: Biostatistical Analysis. 3rd edition. Prentice Hall; New Jersey: 1996

16. 

Lengagne R, Le Gal FA, Garcette M, Fiette L, Ave P, Kato M, Briand JP, Massot C, Nakashima I, Rĕnia L, Guillet JG and Prĕvost-Blondel A: Spontaneous vitiligo in an animal model for human melanoma: Role of tumor-specific CD8+ T cells. Cancer Res. 64:1496–1501. 2004. View Article : Google Scholar : PubMed/NCBI

17. 

Sun HX, Zheng QF and Tu J: Induction of apoptosis in HeLa cells by 3beta-hydroxy-12-oleanen-27-oic acid from the rhizomes of Astilbe chinensis. Bioorg Med Chem. 14:1189–1198. 2006. View Article : Google Scholar : PubMed/NCBI

18. 

Fu J, Bian M, Jiang Q and Zhang C: Roles of Aurora kinases in mitosis and tumorigenesis. Mol Cancer Res. 5:1–10. 2007. View Article : Google Scholar : PubMed/NCBI

19. 

Naruganahalli KS, Lakshmanan M, Dastidar SG and Ray A: Therapeutic potential of Aurora kinase inhibitors in cancer. Curr Opin Investig Drugs. 7:1044–1051. 2006.PubMed/NCBI

20. 

Brittle AL and Ohkura H: Centrosome maturation: Aurora lights the way to the poles. Curr Biol. 15:880–882. 2005. View Article : Google Scholar : PubMed/NCBI

21. 

Taguchi A, Blood DC, del Toro G, Canet A, Lee DC, Qu W, Tanji N, Lu Y, Lalla E, Fu C, Hofmann MA, Kisilinger T, Ingram M, Lu A, Tanaka H, Hori O, Ogawa S, Stern DM and Schmidt AM: Blockade of RAGE-amphoterin signaling suppresses tumor growth and metastases. Nature. 18:354–360. 2000.

22. 

Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellio M, Che J, Frazier A, Yang H, Ivanova S, Borovikova L, Manogue KR, Faist E, Abraham E, Andersson J, Andersson U, Molina PE, Abumrad NN, Sama A and Tracey KJ: HMG-1 as a late mediator of endotoxin lethality in mice. Science. 285:m248–251. 1999. View Article : Google Scholar

23. 

Taniguchi N, Kawahara K, Yone K, Hashiguchi T, Yamakuchi M, Goto M, Inoue K, Yamada S, Ijiri K, Matunaga S, Nakajima T, Komiya S and Maruyama I: High mobility group box chromosomal protein 1, a DNA binding cytokine, induces arthritis. Arthritis Rheum. 48:971–981. 2003. View Article : Google Scholar : PubMed/NCBI

24. 

Ito T, Kawahara K, Okamoto K, Yamada S, Yasuda M, Imaizumi H, Nawa Y, Meng X, Shrestha B, Hashiguchi T and Maruyama I: Proteolytic cleavage of high mobility group box 1 protein by thrombomodulin complexes. Arterioscler Thromb Vasc Biol. 28:1825–1830. 2008. View Article : Google Scholar : PubMed/NCBI

25. 

Inoue K, Kawahara K, Biswas KK, Ando K, Mitsudo K, Nobuyoshi M and Maruyama I: HMGB1 expression by activated vascular smooth muscle cells in advanced human atherosclerosis. Cardiovasc Pathol. 16:136–143. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Matsushita S, Tada K, Kawahara K, Kawai K, Hashiguchi T, Maruyama I and Kanekura T: Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study . Exp Ther Med 1: 569-574, 2010.
APA
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., & Kanekura, T. (2010). Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study . Experimental and Therapeutic Medicine, 1, 569-574. https://doi.org/10.3892/etm_00000089
MLA
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., Kanekura, T."Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study ". Experimental and Therapeutic Medicine 1.4 (2010): 569-574.
Chicago
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., Kanekura, T."Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study ". Experimental and Therapeutic Medicine 1, no. 4 (2010): 569-574. https://doi.org/10.3892/etm_00000089
Copy and paste a formatted citation
x
Spandidos Publications style
Matsushita S, Tada K, Kawahara K, Kawai K, Hashiguchi T, Maruyama I and Kanekura T: Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study . Exp Ther Med 1: 569-574, 2010.
APA
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., & Kanekura, T. (2010). Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study . Experimental and Therapeutic Medicine, 1, 569-574. https://doi.org/10.3892/etm_00000089
MLA
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., Kanekura, T."Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study ". Experimental and Therapeutic Medicine 1.4 (2010): 569-574.
Chicago
Matsushita, S., Tada, K., Kawahara, K., Kawai, K., Hashiguchi, T., Maruyama, I., Kanekura, T."Advanced malignant melanoma responds to Prunus mume Sieb. Et Zucc (Ume) extract: Case report and in vitro study ". Experimental and Therapeutic Medicine 1, no. 4 (2010): 569-574. https://doi.org/10.3892/etm_00000089
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team