Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum

  • Authors:
    • Tomoyuki Tsujikawa
    • Tetsuya Fukunaga
    • Akihiko Itoh
    • Jin Satoh
    • Takashi Yasuoka
    • Masaya Sasaki
    • Yoshihide Fujiyama
    • Tadao Bamba
  • View Affiliations

  • Published online on: October 1, 2002     https://doi.org/10.3892/ijmm.10.4.489
  • Pages: 489-492
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Abstract

The adaptive hyperplasia of the residual intestine after a massive bowel resection is not fully understood. We investigated the alterations in polyamine and glucose-related enzyme mRNA expression during intestinal adaptation. Six-week-old male Wistar rats underwent an 80% resection of the small intestine. The residual ileum was removed on the preoperative day (control) and on postoperative day (POD) 1, 3, 5 and 7. The total RNA was extracted from the mucosa, and a Northern blot analysis was performed. In the residual small intestine, the expression of polyamine synthesis enzymes, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) mRNAs were increased on POD 1. The expression of polyamine degradation enzymes diamine oxidase (DAO) and spermidine/spermine N1-acetyltransferase (SSAT) mRNA did not change dramatically. Antizyme-1 (AZ-1) mRNA was significantly increased on POD 1. The mRNA expression of glucose absorption and metabolism-related proteins, including the Na+-dependent D-glucose cotransporter (SGLT1), fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase (Fru-6-P,2-kinase/Fru-2,6-Pase) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were only slightly changed on POD 1. The enzymes responsible for polyamine biosynthesis but not catabolism were upregulated at the translational level in enterocytes after a small bowel resection. The expression of glucose transport and glycolysis enzyme mRNAs did not increase after a small bowel resection.

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October 2002
Volume 10 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tsujikawa T, Fukunaga T, Itoh A, Satoh J, Yasuoka T, Sasaki M, Fujiyama Y and Bamba T: Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum. Int J Mol Med 10: 489-492, 2002
APA
Tsujikawa, T., Fukunaga, T., Itoh, A., Satoh, J., Yasuoka, T., Sasaki, M. ... Bamba, T. (2002). Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum. International Journal of Molecular Medicine, 10, 489-492. https://doi.org/10.3892/ijmm.10.4.489
MLA
Tsujikawa, T., Fukunaga, T., Itoh, A., Satoh, J., Yasuoka, T., Sasaki, M., Fujiyama, Y., Bamba, T."Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum". International Journal of Molecular Medicine 10.4 (2002): 489-492.
Chicago
Tsujikawa, T., Fukunaga, T., Itoh, A., Satoh, J., Yasuoka, T., Sasaki, M., Fujiyama, Y., Bamba, T."Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum". International Journal of Molecular Medicine 10, no. 4 (2002): 489-492. https://doi.org/10.3892/ijmm.10.4.489