Role of apoptosis inhibition in various chondrocyte culture systems

  • Authors:
    • Toshiaki Takahashi
    • Hiroshi Yamamoto
    • Yasuhiro Ogawa
    • Shoji Yoshida
    • Sayo Kataoka
    • Manas Majumdar
    • Elisabeth Morris
    • Stephen B. Tripple
  • View Affiliations

  • Published online on: March 1, 2003     https://doi.org/10.3892/ijmm.11.3.299
  • Pages: 299-303
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Abstract

Apoptosis may limit the utility of cell-based therapies for articular cartilage disorders. We tested the hypothesis that chondrocyte apoptosis can be reduced by optimizing the conditions employed to expand chondrocyte numbers in culture. Chondrocyte apoptosis was examined in monolayer and suspension culture, in the presence of fetal bovine serum (FBS) or autologous serum, and for culture periods of 2 or 4 days. The effect of these variables was assessed by measuring cell viability, Annexin V labeling and mitochondrial membrane potential. After 2 days of culture, the greatest increase in viable cell number (3.7-fold) occurred in monolayer cultures with autologous serum. After 4 days of culture, the greatest increase in cell number (9.0-fold) occurred in monolayer cultures supplemented with FBS. By Annexin V staining, the proportion of cells undergoing apoptosis after 2 days was not affected by the type of serum used or by culture in monolayer versus suspension. After 4 days of culture, the proportion of apoptotic cells was significantly reduced (35% to 13%, p<0.02) in suspension cultures with autologous serum. Apoptosis assessed by loss of mitochondrial membrane potential was decreased in the presence of autologous serum. These data suggest that suspension culture with autologous serum is useful in simultaneously maintaining cell proliferation and minimizing apoptosis in cultured human articular chondrocytes.

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March 2003
Volume 11 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Takahashi T, Yamamoto H, Ogawa Y, Yoshida S, Kataoka S, Majumdar M, Morris E and Tripple SB: Role of apoptosis inhibition in various chondrocyte culture systems. Int J Mol Med 11: 299-303, 2003
APA
Takahashi, T., Yamamoto, H., Ogawa, Y., Yoshida, S., Kataoka, S., Majumdar, M. ... Tripple, S.B. (2003). Role of apoptosis inhibition in various chondrocyte culture systems. International Journal of Molecular Medicine, 11, 299-303. https://doi.org/10.3892/ijmm.11.3.299
MLA
Takahashi, T., Yamamoto, H., Ogawa, Y., Yoshida, S., Kataoka, S., Majumdar, M., Morris, E., Tripple, S. B."Role of apoptosis inhibition in various chondrocyte culture systems". International Journal of Molecular Medicine 11.3 (2003): 299-303.
Chicago
Takahashi, T., Yamamoto, H., Ogawa, Y., Yoshida, S., Kataoka, S., Majumdar, M., Morris, E., Tripple, S. B."Role of apoptosis inhibition in various chondrocyte culture systems". International Journal of Molecular Medicine 11, no. 3 (2003): 299-303. https://doi.org/10.3892/ijmm.11.3.299