T cell epitopes of type II collagen in HLA-DRB1*0101 or DRB1*0405-positive Japanese patients with rheumatoid arthritis

  • Authors:
    • Yasuyuki Ohnishi
    • Akito Tsutsumi
    • Tateo Sakamaki
    • Takayuki Sumida
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  • Published online on: March 1, 2003     https://doi.org/10.3892/ijmm.11.3.331
  • Pages: 331-335
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Abstract

Rheumatoid arthritis (RA) is a T cell-mediated autoimmune disease, but target antigens (autoantigens) responsible for T cell activation remain unclear. Type II collagen (CII) is a candidate autoantigen that is largely confined to the articular cartilage. To investigate whether CII is an important antigen in patients with RA, we examined peripheral blood T cell reactivity to CII in HLA-DRB1*0101 and DRB1*0405-positive RA patients. Reactivities to candidate T cell epitopes of CII were also examined. Peripheral blood T cell reactivity to CII and CII peptides (256-271, 429-442, 593-610, 1064-1081) were detected by measurement of IL-2, IFN-γ, and IL-4 in culture supernatant of PBMC after in vitro antigen stimulation. Cytokine concentration was measured by ELISA. In DRB1*0101-positive patients, T cell reactivity to CII as detected by measurement of IL-2 production in culture supernatant, was present in 4 out of 9 patients. IL-2 production upon stimulation with CII 256-271 peptide was found in all of these 4 patients. In DRB1*0405-positive patients, high frequency of positive T cell response to CII was detected in 9 out of 11 patients. IFN-γ production was also detected in 4 out of 6 patients producing IL-2 by stimulation with CII. T cell response to CII 256-271 and/or CII 1064-1081 was detected in these patients. In DRB1*0101-positive RA patients, CII 256-271 peptide might function as a T cell epitope, whereas either CII 256-271 or CII 1064-1081 peptide may be a major T cell epitope in DRB1*0405-positive RA patients. In DRB1*0405-positive RA patients, CII reactive T cells might play a crucial role in the development of RA through IFN-γ production.

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March 2003
Volume 11 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ohnishi Y, Tsutsumi A, Sakamaki T and Sumida T: T cell epitopes of type II collagen in HLA-DRB1*0101 or DRB1*0405-positive Japanese patients with rheumatoid arthritis. Int J Mol Med 11: 331-335, 2003
APA
Ohnishi, Y., Tsutsumi, A., Sakamaki, T., & Sumida, T. (2003). T cell epitopes of type II collagen in HLA-DRB1*0101 or DRB1*0405-positive Japanese patients with rheumatoid arthritis. International Journal of Molecular Medicine, 11, 331-335. https://doi.org/10.3892/ijmm.11.3.331
MLA
Ohnishi, Y., Tsutsumi, A., Sakamaki, T., Sumida, T."T cell epitopes of type II collagen in HLA-DRB1*0101 or DRB1*0405-positive Japanese patients with rheumatoid arthritis". International Journal of Molecular Medicine 11.3 (2003): 331-335.
Chicago
Ohnishi, Y., Tsutsumi, A., Sakamaki, T., Sumida, T."T cell epitopes of type II collagen in HLA-DRB1*0101 or DRB1*0405-positive Japanese patients with rheumatoid arthritis". International Journal of Molecular Medicine 11, no. 3 (2003): 331-335. https://doi.org/10.3892/ijmm.11.3.331