Comparison of radiation-induced reactive oxygen species formation in adult articular chondrocytes and that in human peripheral T cells: Possible implication in radiosensitivity

Ogawa,Yasuhiro; Takahashi,Toshiaki; Kobayashi,Toshihiro; Toda,Michio; Nishioka,Akihito; Kariya,Shinji; Seguchi,Harumichu; Yamamoto,Hiroshi; Yoshida,Shoji

doi:10.3892/ijmm.11.4.455

April 2003 Volume 11 Issue 4

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April 2003 Volume 11 Issue 4

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Article

Comparison of radiation-induced reactive oxygen species formation in adult articular chondrocytes and that in human peripheral T cells: Possible implication in radiosensitivity

Authors:
- Yasuhiro Ogawa
- Toshiaki Takahashi
- Toshihiro Kobayashi
- Michio Toda
- Akihito Nishioka
- Shinji Kariya
- Harumichu Seguchi
- Hiroshi Yamamoto
- Shoji Yoshida
View Affiliations / Copyright

Affiliations: Department of Radiology, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi-Prefecture 783-8505, Japan. ogaway@kochi-ms.ac.jp
Pages: 455-459
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Published online on: April 1, 2003

https://doi.org/10.3892/ijmm.11.4.455
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Abstract

Previously, we examined the formation of reactive oxygen species (ROS) in T lymphocytes following 5 Gy of irradiation. Using a CCD camera system, we monitored fluorescence in T lymphocytes loaded with the succinimidyl ester of dichlorodihydrofluorescein diacetate (H2DCFDA), which is non-fluorescent until oxidized by ROS. We found that ROS formation occurred immediately after irradiation, continued for several hours, and resulted in oxidative DNA damage. Therefore, the origin of the hyper-radiosensitivity of T lymphocytes seemed to be the high production of ROS in the mitochondrial DNA following irradiation. In this study, we examined radiation-induced ROS formation in adult articular chondrocytes, which were demonstrated to be highly resistant to apoptosis in our previous study. We found that ROS formation was actually scarcely seen after irradiation of up to 20 Gy in these cells. Therefore, the origin of the great difference of radiosensitivity between T lymphocytes and adult articular chondrocytes is considered to lie in the degree of ROS formation following irradiation, with this difference possibly resulting from the scavenging acuity of these two kinds of normal tissue cells for free radicals including hydroxyl radicals.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparison of radiation-induced reactive oxygen species formation in adult articular chondrocytes and that in human peripheral T cells: Possible implication in radiosensitivity

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