Purification by affinity chromatography of H1° RNA-binding proteins from rat brain

  • Authors:
    • Maria Scaturrok
    • Anna Sala
    • Giuseppe Cutrona
    • Lavinia Raimondi
    • Giuseppe Cannino
    • Simona Fontana
    • Ida Pucci-Minafra
    • Italia Di Liegro
  • View Affiliations

  • Published online on: April 1, 2003     https://doi.org/10.3892/ijmm.11.4.509
  • Pages: 509-513
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Abstract

Post-transcriptional regulation of mRNA metabolism is involved in processes as different as cell fate specification in development and cell response to a large variety of environmental cues. Regulation of all steps of RNA metabolism depends on RNA-binding proteins (RBPs). By using a T1 RNase-protection assay, we previously identified three H1° RNA-binding factors (p40, p70 and p110), highly expressed in the rat brain. Here we report enrichment of these factors from brain extracts, obtained by affinity chromato-graphy of biotinylated H1° RNA-protein complexes on streptavidin-conjugated paramagnetic particles. The purified proteins maintain RNA-binding ability and preference for histone messages.

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April 2003
Volume 11 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Scaturrok M, Sala A, Cutrona G, Raimondi L, Cannino G, Fontana S, Pucci-Minafra I and Di Liegro I: Purification by affinity chromatography of H1° RNA-binding proteins from rat brain. Int J Mol Med 11: 509-513, 2003
APA
Scaturrok, M., Sala, A., Cutrona, G., Raimondi, L., Cannino, G., Fontana, S. ... Di Liegro, I. (2003). Purification by affinity chromatography of H1° RNA-binding proteins from rat brain. International Journal of Molecular Medicine, 11, 509-513. https://doi.org/10.3892/ijmm.11.4.509
MLA
Scaturrok, M., Sala, A., Cutrona, G., Raimondi, L., Cannino, G., Fontana, S., Pucci-Minafra, I., Di Liegro, I."Purification by affinity chromatography of H1° RNA-binding proteins from rat brain". International Journal of Molecular Medicine 11.4 (2003): 509-513.
Chicago
Scaturrok, M., Sala, A., Cutrona, G., Raimondi, L., Cannino, G., Fontana, S., Pucci-Minafra, I., Di Liegro, I."Purification by affinity chromatography of H1° RNA-binding proteins from rat brain". International Journal of Molecular Medicine 11, no. 4 (2003): 509-513. https://doi.org/10.3892/ijmm.11.4.509