Regulation of the cellular subpopulation ratios of normal human endometrial stromal cells by macrophage colony-stimulating factor

  • Authors:
    • Tetsuji Tanaka
    • Naohiko Umesaki
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  • Published online on: May 1, 2003     https://doi.org/10.3892/ijmm.11.5.617
  • Pages: 617-620
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Abstract

Normal human endometrial stromal cells (ESCs) stimulated with 8-Br-cAMP secrete significantly large amounts of PRL and granulocyte colony-stimulating factor (G-CSF), whereas unstimulated stromal cells secreted little. However, there is no relation between PRL and G-CSF levels secreted from the stimulated cells, which suggests that PRL-secreting stromal cells may not completely coincide with the G-CSF-producing stromal cells. Recently we found that macrophage colony-stimulating factor (M-CSF) inhibits 8-Br-cAMP-induced decidualization, differentiation to prolactin (PRL)-secreting cells, by suppressing viable decidualizing cells. Therefore, we investigated the effects of M-CSF on G-CSF secretion from normal human endometrial stromal cells using an in vitro decidualization activity assay. M-CSF did not show any significant effects on viable cell numbers of unstimulated ESCs, while M-CSF dose-dependently enhanced G-CSF release from the non-decidualized stromal cells. A high concentration of M-CSF strongly suppressed the viable cell numbers and PRL secretion of stromal cells co-stimulated with 8-Br-cAMP and M-CSF, although G-CSF release from the co-stimulated stromal cells was not affected by M-CSF. Moreover, M-CSF did not affect viable cell numbers, PRL secretion or G-CSF secretion of 8-Br-cAMP-stimulated cells. These results indicate that M-CSF enhances G-CSF secretion from 8-Br-cAMP-unstimulated human endometrial stromal cells but not from 8-Br-cAMP-stimulated stromal cells, thus suggesting that there exists a functional subpopulation of G-CSF-secreting stromal cells that are different from the predecidualized ESCs that differentiate into PRL-secreting ESCs under stimuli with 8-Br-cAMP. Hence, M-CSF may autoregulate functional cellular subpopulations of human endometrial stromal cells in an autocrine or a paracrine manner.

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May 2003
Volume 11 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanaka T and Tanaka T: Regulation of the cellular subpopulation ratios of normal human endometrial stromal cells by macrophage colony-stimulating factor. Int J Mol Med 11: 617-620, 2003
APA
Tanaka, T., & Tanaka, T. (2003). Regulation of the cellular subpopulation ratios of normal human endometrial stromal cells by macrophage colony-stimulating factor. International Journal of Molecular Medicine, 11, 617-620. https://doi.org/10.3892/ijmm.11.5.617
MLA
Tanaka, T., Umesaki, N."Regulation of the cellular subpopulation ratios of normal human endometrial stromal cells by macrophage colony-stimulating factor". International Journal of Molecular Medicine 11.5 (2003): 617-620.
Chicago
Tanaka, T., Umesaki, N."Regulation of the cellular subpopulation ratios of normal human endometrial stromal cells by macrophage colony-stimulating factor". International Journal of Molecular Medicine 11, no. 5 (2003): 617-620. https://doi.org/10.3892/ijmm.11.5.617