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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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August 2003 Volume 12 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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August 2003 Volume 12 Issue 2

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Article

Cloning and characterization of a novel splicing isoform of USF1

  • Authors:
    • Tomoko Saito
    • Takayuki Oishi
    • Kazuyuki Yanai
    • Yoko Shimamoto
    • Akiyoshi Fukamizu
  • View Affiliations / Copyright

    Affiliations: Institute of Applied Biochemistry, Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan
  • Pages: 161-167
    |
    Published online on: August 1, 2003
       https://doi.org/10.3892/ijmm.12.2.161
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Abstract

The ubiquitous basic helix-loop-helix transcription factor USFs encoded by two distinct genes (USF1 and USF2) recognize a core motif, CACGTG, termed E box and regulate the expression of a variety of genes. USF1 and USF2 proteins form homo- and heterodimers to bind the target core motif DNA. Here, we report the molecular cloning and functional characterization of a novel alternative splicing variant of human USF1 (hUSF1), termed USF1/BD. Compared with USF1 wild-type (wt), USF1/BD lacks the N-terminal transactivation domain. Cloning and characterization of the hUSF1 genomic region revealed that USF1/BD is generated by excising the sequence corresponding to a part of exon 4. In transiently transfected cells, USF1/BD was localized in the nucleus and repressed the promoter activity of the human angiotensinogen gene. In vitro translated USF1/BD possessed DNA binding activity as a homodimer and a heterodimer with USF1 (wt). These results suggest that USF1/BD plays a role as a modulator of USF1 to control the expression of target genes.

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Copy and paste a formatted citation
Spandidos Publications style
Saito T, Oishi T, Yanai K, Shimamoto Y and Fukamizu A: Cloning and characterization of a novel splicing isoform of USF1. Int J Mol Med 12: 161-167, 2003.
APA
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., & Fukamizu, A. (2003). Cloning and characterization of a novel splicing isoform of USF1. International Journal of Molecular Medicine, 12, 161-167. https://doi.org/10.3892/ijmm.12.2.161
MLA
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., Fukamizu, A."Cloning and characterization of a novel splicing isoform of USF1". International Journal of Molecular Medicine 12.2 (2003): 161-167.
Chicago
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., Fukamizu, A."Cloning and characterization of a novel splicing isoform of USF1". International Journal of Molecular Medicine 12, no. 2 (2003): 161-167. https://doi.org/10.3892/ijmm.12.2.161
Copy and paste a formatted citation
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Spandidos Publications style
Saito T, Oishi T, Yanai K, Shimamoto Y and Fukamizu A: Cloning and characterization of a novel splicing isoform of USF1. Int J Mol Med 12: 161-167, 2003.
APA
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., & Fukamizu, A. (2003). Cloning and characterization of a novel splicing isoform of USF1. International Journal of Molecular Medicine, 12, 161-167. https://doi.org/10.3892/ijmm.12.2.161
MLA
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., Fukamizu, A."Cloning and characterization of a novel splicing isoform of USF1". International Journal of Molecular Medicine 12.2 (2003): 161-167.
Chicago
Saito, T., Oishi, T., Yanai, K., Shimamoto, Y., Fukamizu, A."Cloning and characterization of a novel splicing isoform of USF1". International Journal of Molecular Medicine 12, no. 2 (2003): 161-167. https://doi.org/10.3892/ijmm.12.2.161
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