Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array

  • Authors:
    • Keisuke Aoe
    • Akio Hiraki
    • Tomoyuki Murakami
    • Kazuo Murakami
    • Kiyoshi Makihata
    • Kazushi Takao
    • Ryosuke Eda
    • Tadashi Maeda
    • Kazuro Sugi
    • Zbigniew Darzynkiewicz
    • Hiroyasu Takeyama
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijmm.12.2.193
  • Pages: 193-198
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Abstract

Several cytokines play significant roles in the development and pathogenesis of pleural effusion. Little is known, however, about possible interactions between individual cytokines in terms of regulation of their relative abundance in the effusion. We studied 93 patients presenting with pleural effusion to the National Sanyo Hospital (68 men and 25 women; mean age, 64 years). Twenty-two patients had tuberculous pleurisy, 40 had malignant pleuritis, and 31 had effusions due to an etiology other than tuberculosis or cancer (miscellaneous). Pleural fluid concentrations of IL-2, IL-4, IL-5, IL-10, TNF-α, and INF-γ were simultaneously measured by cytometric bead array (CBA). The ratios of IL-4/IL-5, IL-4/TNF-α, IL-2/TNF-α, and IL-10/TNF-α were lower in patients with tuberculosis pleurisy compared with other patients. In all three groups of patients significant correlation was seen between abundance of IL-2 vs. IL-4, IL-5, IL-10, or TNF-α, between IL-4 vs. IL-10, and between TNF-α vs. INF-γ. In malignant pleural fluid patients, the significant correlation was between IL-2 vs. IL-4, TNF-α, or INF-γ, between IL-4 vs. INF-γ, and between TNF-α vs. INF-γ. In tuberculosis pleural fluid patients, the significant correlation was between IL-2 vs. TNF-α, between IL-4 vs. IL-10, and between TNF-α vs. INF-γ. In miscellaneous pleural fluid patients, the significant correlation was between IL-2 vs. IL-4, IL-10, or TNF-α, between IL-4 vs. IL-10, TNF-α, and between IL-10 vs. TNF-α. No significant correlation was observed between other pairs of cytokines. Strong correlation in abundance between particular cytokines in pleural fluids suggests cross-talk between them, in terms that an altered level of one of them provides a feedback mechanism regulating synthesis and/or secretion of another one. Such interactions may play important roles in pathogenesis and severity of the effusion. The CBA methodology provides a convenient tool to investigate these interactions.

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August 2003
Volume 12 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Aoe K, Hiraki A, Murakami T, Murakami K, Makihata K, Takao K, Eda R, Maeda T, Sugi K, Darzynkiewicz Z, Darzynkiewicz Z, et al: Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array. Int J Mol Med 12: 193-198, 2003.
APA
Aoe, K., Hiraki, A., Murakami, T., Murakami, K., Makihata, K., Takao, K. ... Takeyama, H. (2003). Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array. International Journal of Molecular Medicine, 12, 193-198. https://doi.org/10.3892/ijmm.12.2.193
MLA
Aoe, K., Hiraki, A., Murakami, T., Murakami, K., Makihata, K., Takao, K., Eda, R., Maeda, T., Sugi, K., Darzynkiewicz, Z., Takeyama, H."Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array". International Journal of Molecular Medicine 12.2 (2003): 193-198.
Chicago
Aoe, K., Hiraki, A., Murakami, T., Murakami, K., Makihata, K., Takao, K., Eda, R., Maeda, T., Sugi, K., Darzynkiewicz, Z., Takeyama, H."Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array". International Journal of Molecular Medicine 12, no. 2 (2003): 193-198. https://doi.org/10.3892/ijmm.12.2.193