In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines

  • Authors:
    • Tetsuhiro Moriya
    • Naohiko Seki
    • Ken Shimada
    • Masaki Kato
    • Takashi Yakushiji
    • Yoshinori Nimura
    • Katsuhiro Uzawa
    • Masaki Takiguchi
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijmm.12.4.429
  • Pages: 429-435
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Abstract

To analyze gene expression in oral cancer, we produced a specialized in-house cDNA microarray. The cDNA library was constructed from surgical specimens of oral squamous cell carcinoma (SCC) using an oligo-capping method. cDNA clones (n=4,800) were randomly selected and their 5'-end nucleotide sequences were determined. Overlapping clones were excluded, and 1,423 independent clones were selected and used for microarray production. Compared to the public nucleotide sequence database, 61% of our cDNA clones were full-length. By correlating expression patterns across SCC cell lines, we identified 53 genes (7 up-regulated and 46 down-regulated) that are differentially expressed in SCC cell lines compared to normal mucosa. Semi-quantitative RT-PCR analysis confirmed these findings and validity of our cDNA microarray. Using specimens from SCC patients, we investigated the expression status of the IL-1ra gene, which showed the down-regulated gene by microarray analysis. Gene expression clearly fell in the SCC specimens relative to their references, which indicated that our in-house cDNA microarray system rapidly identified and characterized candidate biomarkers for clinical use.

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October 2003
Volume 12 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Moriya T, Seki N, Shimada K, Kato M, Yakushiji T, Nimura Y, Uzawa K, Takiguchi M and Tanzawa H: In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines. Int J Mol Med 12: 429-435, 2003
APA
Moriya, T., Seki, N., Shimada, K., Kato, M., Yakushiji, T., Nimura, Y. ... Tanzawa, H. (2003). In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines. International Journal of Molecular Medicine, 12, 429-435. https://doi.org/10.3892/ijmm.12.4.429
MLA
Moriya, T., Seki, N., Shimada, K., Kato, M., Yakushiji, T., Nimura, Y., Uzawa, K., Takiguchi, M., Tanzawa, H."In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines". International Journal of Molecular Medicine 12.4 (2003): 429-435.
Chicago
Moriya, T., Seki, N., Shimada, K., Kato, M., Yakushiji, T., Nimura, Y., Uzawa, K., Takiguchi, M., Tanzawa, H."In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines". International Journal of Molecular Medicine 12, no. 4 (2003): 429-435. https://doi.org/10.3892/ijmm.12.4.429