Effects of progestins of human proliferative endometrium: An in vitro model of potential clinical relevance

  • Authors:
    • Séverine Illouz
    • Jean-Philippe Dales
    • Karine Sferlazzo
    • Stéphane Garcia
    • Séverine Carpentier-Meunier
    • Léon Boubli
    • Marie-Noelle Lavaut
    • Colette Charpin
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijmm.12.4.517
  • Pages: 517-523
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Abstract

Endometrium biopsy is a useful indicator of endometrium proliferation and is clinically relevant to diagnose cell proliferation and to evaluate response to progestin treatment and to monitor hormone replacement therapy. The aim of our study was to investigate the in vitro effects of progesterone and synthetic progestins on endometrium explants with a particular focus on estradiol receptor (ER) and progesterone receptor (PR) expression which reflects through cell secretion the hormone treatment efficiency. Most widely used progestagens belonging to three disctintive groups were investigated, i.e, medroxyprogesterone acetate (MPA), norethindrone acetate (NOR) and nomegestrol acetate (TX) which are respectively pregnane, 19-nortestosterone and norpregnane derivatives. We used organ culture from human proliferative endometrium, in which tissue integrity, particulary gland/stroma relationships are preserved. Progestins induce epithelial cell secretion and most effects were observed at the highest concentration tested (10−7 M) and by TX and MPA on homogeneous and on heterogeneous (including also secretory glands) proliferative endometrium respectively. In these conditions, ER as well as PR expression were decreased on both glandular and stromal cells. In contrast, progesterone at 10−7 M significantly decreased only PR, in glands and in stroma of homogeneous proliferative endometrium, and just in stroma of heterogeneous endometrium. NOR exhibited less effects. At lower concentrations (10−8 M, 10−9 M), significantly less effects were observed by synthetic progestins on proliferative endometrium. The experiments show that the different types of progestins do not exhibit in vitro similar effects. Since progestins variably act on proliferative endometrium, the exposure of endometrium explants to progestins may be a useful tool to predict clinical response to hormone therapy (individual ‘hormonogram’) and to monitor endometrium proliferation.

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October 2003
Volume 12 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Illouz S, Dales J, Sferlazzo K, Garcia S, Carpentier-Meunier S, Boubli L, Lavaut M and Charpin C: Effects of progestins of human proliferative endometrium: An in vitro model of potential clinical relevance. Int J Mol Med 12: 517-523, 2003.
APA
Illouz, S., Dales, J., Sferlazzo, K., Garcia, S., Carpentier-Meunier, S., Boubli, L. ... Charpin, C. (2003). Effects of progestins of human proliferative endometrium: An in vitro model of potential clinical relevance. International Journal of Molecular Medicine, 12, 517-523. https://doi.org/10.3892/ijmm.12.4.517
MLA
Illouz, S., Dales, J., Sferlazzo, K., Garcia, S., Carpentier-Meunier, S., Boubli, L., Lavaut, M., Charpin, C."Effects of progestins of human proliferative endometrium: An in vitro model of potential clinical relevance". International Journal of Molecular Medicine 12.4 (2003): 517-523.
Chicago
Illouz, S., Dales, J., Sferlazzo, K., Garcia, S., Carpentier-Meunier, S., Boubli, L., Lavaut, M., Charpin, C."Effects of progestins of human proliferative endometrium: An in vitro model of potential clinical relevance". International Journal of Molecular Medicine 12, no. 4 (2003): 517-523. https://doi.org/10.3892/ijmm.12.4.517