Delivery of recombinant adeno-associated virus by jet injection

  • Authors:
    • Olga Janouskova
    • Thomas Nellessen
    • Jitka Stokrova
    • Pavel Jinoch
    • Michal Smahel
  • View Affiliations

  • Published online on: November 1, 2003     https://doi.org/10.3892/ijmm.12.5.687
  • Pages: 687-691
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Abstract

The jet-injection technology was used for delivery of recombinant adeno-associated virus (rAAV). Although AAV-based vectors are an attractive tool in gene therapy, some methodological and technical problems of their targeted delivery remain to be solved. We tried to address some of these cell-targeting problems by using a new low-volume needleless injection device the Swiss Injector. First we tested, by electron microscopy, whether jet-injection would have any detrimental effect on rAAV particle integrity. Second, we compared transgene expression after infection of 293T cells with fired or control (non-fired) rAAV that expressed the green fluorescent protein (GFP), β-galactosidase (β-gal), the B7.1 molecule, and interleukin 2 (IL2). Third, an rAAV carrying the genes coding for β-gal was jet-injected into mouse subcutaneous (s.c.) tumours. The staining of tumour cryosections revealed β-gal expression 72 h after the delivery. Our study demonstrated the applicability of the Swiss Injector for the delivery of rAAV into tumour tissue without either vector particle integrity or the level of expression of the transgenes, as tested in vitro, being affected. The jet-injection technology could improve the distribution of vector particles in the tumour mass without leakage of liquid from the injection site.

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November 2003
Volume 12 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Janouskova O, Nellessen T, Stokrova J, Jinoch P and Smahel M: Delivery of recombinant adeno-associated virus by jet injection. Int J Mol Med 12: 687-691, 2003
APA
Janouskova, O., Nellessen, T., Stokrova, J., Jinoch, P., & Smahel, M. (2003). Delivery of recombinant adeno-associated virus by jet injection. International Journal of Molecular Medicine, 12, 687-691. https://doi.org/10.3892/ijmm.12.5.687
MLA
Janouskova, O., Nellessen, T., Stokrova, J., Jinoch, P., Smahel, M."Delivery of recombinant adeno-associated virus by jet injection". International Journal of Molecular Medicine 12.5 (2003): 687-691.
Chicago
Janouskova, O., Nellessen, T., Stokrova, J., Jinoch, P., Smahel, M."Delivery of recombinant adeno-associated virus by jet injection". International Journal of Molecular Medicine 12, no. 5 (2003): 687-691. https://doi.org/10.3892/ijmm.12.5.687