Manumycin induces apoptosis in human hepatocellular carcinoma HepG2 cells

  • Authors:
    • Jun-Min Zhou
    • Xiao-Feng Zhu
    • Qi-Chao Pan
    • Duan-Fang Liao
    • Zhi-Ming Li
    • Zong-Chao Liu
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijmm.12.6.955
  • Pages: 955-959
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Abstract

Farnesyltransferase inhibitors (FTIs) were developed to prevent Ras processing and thus to be effective agents for the treatment of cancers harbouring mutated ras. In the present study, HepG2 cells underwent internucleosomal DNA fragmentation after treatment with farnesyltransferase inhibitor manumycin (20 µM) for 12 h. Flow cytometric analysis showed that HepG2 cells were accumulated in the G2/M phase of the cell cycle and the number of apoptotic sub-G1 fraction of cells was increased after treatment with manumycin in a time-dependent manner. During the induction of apoptosis, expression of p53 and p21WAF1 was upregulated, phosphorylation of IκB-α was blocked, caspase substrates poly(ADP-ribose) polymerase (PARP) and lamin B were cleaved, and Bcl-2 and Bax protein expression remained unchanged. These results indicated that manumycin induced apoptosis in HepG2 cells. The induction of apoptosis by manumycin involved the upregulation of p53 and p21WAF1, the activation of caspases, and the inhibition of nuclear factor-κB (NF-κB) pathway. However, Bcl-2 and Bax are not associated with manumycin-mediated apoptosis.

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December 2003
Volume 12 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhou J, Zhu X, Pan Q, Liao D, Li Z and Liu Z: Manumycin induces apoptosis in human hepatocellular carcinoma HepG2 cells. Int J Mol Med 12: 955-959, 2003
APA
Zhou, J., Zhu, X., Pan, Q., Liao, D., Li, Z., & Liu, Z. (2003). Manumycin induces apoptosis in human hepatocellular carcinoma HepG2 cells. International Journal of Molecular Medicine, 12, 955-959. https://doi.org/10.3892/ijmm.12.6.955
MLA
Zhou, J., Zhu, X., Pan, Q., Liao, D., Li, Z., Liu, Z."Manumycin induces apoptosis in human hepatocellular carcinoma HepG2 cells". International Journal of Molecular Medicine 12.6 (2003): 955-959.
Chicago
Zhou, J., Zhu, X., Pan, Q., Liao, D., Li, Z., Liu, Z."Manumycin induces apoptosis in human hepatocellular carcinoma HepG2 cells". International Journal of Molecular Medicine 12, no. 6 (2003): 955-959. https://doi.org/10.3892/ijmm.12.6.955