Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers

Leirdal,Marianne; Shadidy,Mohsen; Røsok,Øystein; Sioud,Mouldy

doi:10.3892/ijmm.14.2.217

August 2004 Volume 14 Issue 2

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August 2004 Volume 14 Issue 2

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Article

Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers

Authors:
- Marianne Leirdal
- Mohsen Shadidy
- Øystein Røsok
- Mouldy Sioud
View Affiliations / Copyright

Affiliations: Institute for cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
Pages: 217-222
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Published online on: August 1, 2004

https://doi.org/10.3892/ijmm.14.2.217
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Abstract

The identification of differentially expressed genes in tumour cells should have important implications in understanding carcinogenesis and developing new therapeutic and prognostic biomarkers. We have combined PCR-based cDNA subtraction and Northern blotting to identify truly differentially expressed genes in breast cancer cell line SKBR3 as compared to normal human mammary epithelial cells (HMEC). Hybridizing probe molecules were rescued from the Hybond N+ membranes and then PCR reamplified. The PCR reamplification is possible due to the fact that all probe molecules contain the same pair of adapter sequences on both ends. After cloning and sequencing three known genes, ribosomal protein L19 (RPL19), ADP/ATP carrier protein and ErbB-2 with high-elevated mRNA levels in SKBR3 were identified. In addition, two overexpressed genes with unknown functions, CXYorf1-related protein and hypothetical protein PRO2605, were found. High-titer andibodies against the recombinant RPL19 were detected in 5 patients out of 50 patients investigated. Thus, the present novel strategy based on the combination of PCR-based cDNA subtraction and Northern blotting should facilitate the identification of truly differentially expressed biomarkers, which may offer the potential to determine the proper drug for an individual patient at a given stage of disease or treatment.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers

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