Bone loss in regucalcin transgenic rats: Enhancement of osteoclastic cell formation from bone marrow of rats with increasing age

  • Authors:
    • Satoshi Uchiyama
    • Masayoshi Yamaguchi
  • View Affiliations

  • Published online on: September 1, 2004     https://doi.org/10.3892/ijmm.14.3.451
  • Pages: 451-455
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Abstract

Bone loss was previously shown to be induced in the femoral tissue of regucalcin transgenic (TG) rats. Regucalcin is expressed in rat bone marrow cells and its expression is enhanced in regucalcin TG rats. This study was undertaken to determine the change in osteoclastic bone resorption in regucalcin TG rats with increasing age. Femoral-diaphyseal and -metaphyseal tissues were obtained from normal (wild-type) and regucalcin TG rats aged 5, 14, 25 or 50 weeks. Calcium content in the femoral-diaphyseal and -metaphyseal tissues was significantly decreased in regucalcin TG male and female rats aged 5, 14, 25 or 50 weeks as compared with the value obtained from normal rats with each age. When the marrow cells obtained from normal or regucalcin TG rats were cultured for 7 days, the number of tartrate-resistant acid phosphatase (TRACP), a marker of osteoclasts, positive multinucleated cells (MNCs) were significantly increased in the marrow culture of regucalcin TG male and female rats aged 5, 14, 25 or 50 weeks. The effect of parathyroid hormone [human PTH (1-34); 10−7 M] or 1,25-dihydroxyvitamin D3 [1,25(OH)2D3; 10−7 M] in stimulating TRACP-positive MNC formation was significantly enhanced in regucalcin TG male and female rats aged 14 or 25 weeks. This study demonstrates that osteoclastic bone resorption is stimulated in regucalcin TG male and female rats with increasing age.

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September 2004
Volume 14 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Uchiyama S and Uchiyama S: Bone loss in regucalcin transgenic rats: Enhancement of osteoclastic cell formation from bone marrow of rats with increasing age. Int J Mol Med 14: 451-455, 2004
APA
Uchiyama, S., & Uchiyama, S. (2004). Bone loss in regucalcin transgenic rats: Enhancement of osteoclastic cell formation from bone marrow of rats with increasing age. International Journal of Molecular Medicine, 14, 451-455. https://doi.org/10.3892/ijmm.14.3.451
MLA
Uchiyama, S., Yamaguchi, M."Bone loss in regucalcin transgenic rats: Enhancement of osteoclastic cell formation from bone marrow of rats with increasing age". International Journal of Molecular Medicine 14.3 (2004): 451-455.
Chicago
Uchiyama, S., Yamaguchi, M."Bone loss in regucalcin transgenic rats: Enhancement of osteoclastic cell formation from bone marrow of rats with increasing age". International Journal of Molecular Medicine 14, no. 3 (2004): 451-455. https://doi.org/10.3892/ijmm.14.3.451