Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase.

  • Authors:
    • Hideki Yamashita
    • Mitsuhiko Osaki
    • Tonang Dwi Ardyanto
    • Mari Osaki
    • Haruhiko Yoshida
    • Hisao Ito
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.4.565
  • Pages: 565-635
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Abstract

Cyclooxygenase-2 (COX-2) has been found to be up-regulated in several types of human malignant tumors and proposed to have a role in the angiogenic process. This study examined the expression of COX-2 in two human malignant fibrous histiocytoma (MFH) cell lines by Western blotting, which showed a specific single band at 72 kDa. Immunohistochemistry was conducted in 35 MFHs and 30 benign fibrohistiocytic tumors (BFHTs), comparing the expression of vascular endothelial growth factor (VEGF) and thymidine phosphorylase (TP), as well as intratumoral microvessel density (IMVD). COX-2 expression was noted in 22 (62.9%) MFHs, but in no BFHT. IMVD values were significantly higher in the MFHs (90.6+/-8.0) than BFHTs (27.9+/-3.1), and also in the COX-2 positive (104.5+/-11.3) than negative (67.2+/-5.8) MFHs. VEGF and TP expression was also associated with a significantly higher level of COX-2, as well as greater IMVD. The highest IMVD values were noted in the 17 MFHs (120.8+/-11.5) expressing all three factors. Clinical analysis demonstrated poorer survival in the 18 COX-2 positive MFHs than in the 10 negative ones, although the small number of cases did not reveal a significant difference. The results overall indicated that COX-2 expression is associated with intratumoral angiogenesis, which might provide favorable conditions for tumor progression in human MFHs.

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October 2004
Volume 14 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yamashita H, Osaki M, Ardyanto T, Osaki M, Yoshida H and Ito H: Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase.. Int J Mol Med 14: 565-635, 2004
APA
Yamashita, H., Osaki, M., Ardyanto, T., Osaki, M., Yoshida, H., & Ito, H. (2004). Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase.. International Journal of Molecular Medicine, 14, 565-635. https://doi.org/10.3892/ijmm.14.4.565
MLA
Yamashita, H., Osaki, M., Ardyanto, T., Osaki, M., Yoshida, H., Ito, H."Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase.". International Journal of Molecular Medicine 14.4 (2004): 565-635.
Chicago
Yamashita, H., Osaki, M., Ardyanto, T., Osaki, M., Yoshida, H., Ito, H."Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase.". International Journal of Molecular Medicine 14, no. 4 (2004): 565-635. https://doi.org/10.3892/ijmm.14.4.565