Analysis of the PTCH1 signaling pathway in ovarian dermoids

  • Authors:
    • Sonja Levanat
    • Roland Kappler
    • Bernhard Hemmerlein
    • Patrick Döring
    • Vesna Musani
    • Arijana Komar
    • Slavko Oreskovic
    • Bozidar Pavelic
    • Heidi Hahn
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijmm.14.5.793
  • Pages: 793-799
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Abstract

Dermoids belong to the group of developmental cysts and arise from germ cells. Studies on these tumors may therefore increase our understanding of normal germ cell development within different environments and cell lines derived from these lesions may also constitute an important vehicle for studying neoplasia and differentiation. Recently, we investigated the status of the PTCH1 locus in a large set of sporadic non-inflammatory, developmental cystic lesions. Our data showed allelic loss of microsatellite markers in close vicinity to the PTCH1 locus in both odontogenic keratocysts and dentigerous cysts as well as in ovarian dermoid cysts (ODC). In this study, we closely examined the status of the PTCH1 gene in ODCs. Although about 25% of cysts demonstrated LOH at the PTCH1 locus, no nonsense or missense mutations in the coding region of PTCH1 were detected in genomic DNA isolated from any of the ODCs examined by direct sequencing. Staining with PTCH1 and GLI1 antibodies showed that proteins were present in virtually all epithelial linings, with variable staining intensity not correlated with LOH and generally weaker for GLI1. However, cDNA microarray analysis performed on cell lines derived from ODCs did not show any significant alteration in the expression of the analyzed target genes of PTCH1 signaling in any of the cell lines examined, except for CyclinD1 (and several other genes generally not associated with PTCH1 signaling).

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November 2004
Volume 14 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Levanat S, Kappler R, Hemmerlein B, Döring P, Musani V, Komar A, Oreskovic S, Pavelic B and Hahn H: Analysis of the PTCH1 signaling pathway in ovarian dermoids. Int J Mol Med 14: 793-799, 2004
APA
Levanat, S., Kappler, R., Hemmerlein, B., Döring, P., Musani, V., Komar, A. ... Hahn, H. (2004). Analysis of the PTCH1 signaling pathway in ovarian dermoids. International Journal of Molecular Medicine, 14, 793-799. https://doi.org/10.3892/ijmm.14.5.793
MLA
Levanat, S., Kappler, R., Hemmerlein, B., Döring, P., Musani, V., Komar, A., Oreskovic, S., Pavelic, B., Hahn, H."Analysis of the PTCH1 signaling pathway in ovarian dermoids". International Journal of Molecular Medicine 14.5 (2004): 793-799.
Chicago
Levanat, S., Kappler, R., Hemmerlein, B., Döring, P., Musani, V., Komar, A., Oreskovic, S., Pavelic, B., Hahn, H."Analysis of the PTCH1 signaling pathway in ovarian dermoids". International Journal of Molecular Medicine 14, no. 5 (2004): 793-799. https://doi.org/10.3892/ijmm.14.5.793