Analysis of abnormally expressed genes in synovium from patients with rheumatoid arthritis using a column gel electrophoresis-coupled subtractive hybridization technique

  • Authors:
    • Takashi Tomoo
    • Akito Tsutsumi
    • Takanori Yasukochi
    • Kotaro Ikeda
    • Naoyuki Ochiai
    • Kazuo Ozawa
    • Yasuhiko Shibanaka
    • Satoshi Ito
    • Isao Matsumoto
    • Daisuke Goto
    • Takayuki Sumida
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijmm.15.3.453
  • Pages: 453-457
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Abstract

Rheumatoid arthritis (RA) is a chronic disease of unknown pathogenesis. To identify abnormally expressed genes in synovium from RA patients, we performed column gel electrophoresis-coupled subtractive hybridization (CGESH). CGESH is a newly developed subtractive hybridization technique to achieve sufficient enrichment of DNA sequences. CGESH was performed using restricted enzyme digested cDNA synthesized from mRNA of synovial tissues from one RA patient and one osteoarthritis (OA) patient. The obtained subtraction libraries (RA-OA) were screened by dot blot hybridization. The clones showing higher hybridization with the RA-OA probe were identified by sequence analysis and homology search. Their DNA sequencing revealed that the genes of HLA-DRB1, sequestosome 1, elongation factor 1α were included. Furthermore, a functionally unknown gene (FLJ00133) was also identified. It is reported that sequestosome 1 is a scaffold in the signal transduction of TNFα and interleukin 1, which are the important cytokines involved in the pathogenesis of RA. It is possible that other genes identified by the CGESH technique would be associated with the pathogenesis of RA, although there is no direct evidence yet. Our results imply that the CGESH technique is a useful tool to detect genes involved in the pathogenesis of RA. Further investigation of the functional roles of candidate genes should shed light on the pathogenesis of RA.

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March 2005
Volume 15 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tomoo T, Tsutsumi A, Yasukochi T, Ikeda K, Ochiai N, Ozawa K, Shibanaka Y, Ito S, Matsumoto I, Goto D, Goto D, et al: Analysis of abnormally expressed genes in synovium from patients with rheumatoid arthritis using a column gel electrophoresis-coupled subtractive hybridization technique. Int J Mol Med 15: 453-457, 2005
APA
Tomoo, T., Tsutsumi, A., Yasukochi, T., Ikeda, K., Ochiai, N., Ozawa, K. ... Sumida, T. (2005). Analysis of abnormally expressed genes in synovium from patients with rheumatoid arthritis using a column gel electrophoresis-coupled subtractive hybridization technique. International Journal of Molecular Medicine, 15, 453-457. https://doi.org/10.3892/ijmm.15.3.453
MLA
Tomoo, T., Tsutsumi, A., Yasukochi, T., Ikeda, K., Ochiai, N., Ozawa, K., Shibanaka, Y., Ito, S., Matsumoto, I., Goto, D., Sumida, T."Analysis of abnormally expressed genes in synovium from patients with rheumatoid arthritis using a column gel electrophoresis-coupled subtractive hybridization technique". International Journal of Molecular Medicine 15.3 (2005): 453-457.
Chicago
Tomoo, T., Tsutsumi, A., Yasukochi, T., Ikeda, K., Ochiai, N., Ozawa, K., Shibanaka, Y., Ito, S., Matsumoto, I., Goto, D., Sumida, T."Analysis of abnormally expressed genes in synovium from patients with rheumatoid arthritis using a column gel electrophoresis-coupled subtractive hybridization technique". International Journal of Molecular Medicine 15, no. 3 (2005): 453-457. https://doi.org/10.3892/ijmm.15.3.453