Semaphorin 4A induces growth cone collapse of hippocampal neurons in a Rho/Rho-kinase-dependent manner

Yukawa,Kazunori; Tanaka,Tetsuji; Bai,Tao; Ueyama,Takashi; Owada-Makabe,Kyoko; Tsubota,Yuji; Maeda,Masanobu; Suzuki,Kazuhiro; Kikutani,Hitoshi; Kumanogoh,Atsushi

doi:10.3892/ijmm.16.1.115

July 2005 Volume 16 Issue 1

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July 2005 Volume 16 Issue 1

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Article

Semaphorin 4A induces growth cone collapse of hippocampal neurons in a Rho/Rho-kinase-dependent manner

Authors:
- Kazunori Yukawa
- Tetsuji Tanaka
- Tao Bai
- Takashi Ueyama
- Kyoko Owada-Makabe
- Yuji Tsubota
- Masanobu Maeda
- Kazuhiro Suzuki
- Hitoshi Kikutani
- Atsushi Kumanogoh
View Affiliations / Copyright

Affiliations: Department of Physiology, Wakayama Medical University, Wakayama 641-8509, Japan. kazu59@wakayama-med.ac.jp
Pages: 115-118
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Published online on: July 1, 2005

https://doi.org/10.3892/ijmm.16.1.115
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Abstract

Semaphorins are a family of secreted and membrane-bound proteins, known to control axonal pathfinding. It was recently demonstrated that Semaphorin 4A (Sema4A) is crucially involved in immune cell activation. However, the role of Sema4A in the nervous system has not yet been clarified. To examine if Sema4A can function as a chemo-repulsive cue to growth cones of developing hippocampal neurons, a growth cone collapse assay with recombinant Sema4A was performed in primary hippocampal neurons cultured from E17 mice. In these primary hippocampal neurons, Sema4A induced a significant growth cone collapse as compared with the culture without Sema4A. The Sema4A-induced growth cone collapse could be blocked by Y-27632, a Rho-kinase inhibitor. Furthermore, immunocytochemical analysis with antibodies against Sema4A demonstrated the binding of recombinant Sema4A to the growth cones of hippocampal neurons. Thus, our data indicated that Sema4A could function as a chemo-repulsive cue by activating a receptor whose signal is transmitted to Rho-kinase and induced growth cone collapse of hippocampal neurons.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Semaphorin 4A induces growth cone collapse of hippocampal neurons in a Rho/Rho-kinase-dependent manner

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