Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection

  • Authors:
    • Lewis F. Neville
    • Yoav Barnea
    • Orly Hammer-Munz
    • Eyal Gur
    • Boris Kuzmenko
    • Hamutal Kahel-Raifer
    • Rachel Eren
    • Adi Elkeles
    • Kanneganti G.K. Murthy
    • Csaba Szabó
    • Andrew L. Salzman
    • Shlomo Dagan
    • Yehuda Carmeli
    • Shiri Navon-Venezia
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijmm.16.1.165
  • Pages: 165-171
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Abstract

The goal of this study was to investigate if antibodies raised against N'-terminal Pseudomonas aeruginosa (Pa) flagellin could afford protection in two lethal mouse models of Pa infection. To that end, rabbit polyclonal antibodies were generated against the N'-terminal domains (amino acids 1-156) of recombinant Pa01 or Salmonella muenchen flagellins, termed anti-N'-fla-b and anti-N'-fla-Sm, respectively. In vitro, anti-N'-fla-b but not anti-N'-fla-Sm IgG specifically recognized recombinant and Pa endogenous flagellin type b proteins, total bacterial lysates of Pa type b, and inhibited Pa01 invasion into A549 cells. In vivo, administration of anti-N'-fla-b afforded a remarkable improvement in survival in lethal peritonitis (90% vs. 12% in control; p<0.001) and burn infection (83% vs. 8-17% in control groups; p<0.005) Pa models. These findings would suggest that the N'-terminal domain of Pa flagellin harbors critically important bioactive domains and that an antibody-targeted, neutralization approach directed at this region could provide a novel therapeutic strategy to combat Pa infection.

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July 2005
Volume 16 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Neville LF, Barnea Y, Hammer-Munz O, Gur E, Kuzmenko B, Kahel-Raifer H, Eren R, Elkeles A, Murthy KG, Szabó C, Szabó C, et al: Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection. Int J Mol Med 16: 165-171, 2005
APA
Neville, L.F., Barnea, Y., Hammer-Munz, O., Gur, E., Kuzmenko, B., Kahel-Raifer, H. ... Navon-Venezia, S. (2005). Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection. International Journal of Molecular Medicine, 16, 165-171. https://doi.org/10.3892/ijmm.16.1.165
MLA
Neville, L. F., Barnea, Y., Hammer-Munz, O., Gur, E., Kuzmenko, B., Kahel-Raifer, H., Eren, R., Elkeles, A., Murthy, K. G., Szabó, C., Salzman, A. L., Dagan, S., Carmeli, Y., Navon-Venezia, S."Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection". International Journal of Molecular Medicine 16.1 (2005): 165-171.
Chicago
Neville, L. F., Barnea, Y., Hammer-Munz, O., Gur, E., Kuzmenko, B., Kahel-Raifer, H., Eren, R., Elkeles, A., Murthy, K. G., Szabó, C., Salzman, A. L., Dagan, S., Carmeli, Y., Navon-Venezia, S."Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection". International Journal of Molecular Medicine 16, no. 1 (2005): 165-171. https://doi.org/10.3892/ijmm.16.1.165