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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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December 2005 Volume 16 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells

  • Authors:
    • Karlin R. Karlmark
    • Angelika Freilinger
    • Erika Marton
    • Margit Rosner
    • Gert Lubec
    • Markus Hengstschläger

  • View Affiliations / Copyright

    Affiliations: Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, 1090 Vienna, Austria
  • Pages: 987-992
    |
    Published online on: December 1, 2005
       https://doi.org/10.3892/ijmm.16.6.987
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Abstract

Recently, amniotic fluid was suggested as a new source for stem-cell research and tissue engineering approaches. In order to enable isolation of stem cells and establishment of lines of such cells with an undifferentiated phenotype we have introduced green fluorescent protein regulated by the promoters of the stem cell-specific genes, Oct-4 or Rex-1, into human amniotic fluid cells. For the introduction of DNA into human amniotic fluid cells, we have optimized a specific transfection protocol. We found that human amniotic fluid contains cell populations which are able to activate these promoters. These undifferentiated cells expressing green fluorescent protein can be analysed on a flow cytometer. In addition, we have introduced a plasmid harboring a neomycin-resistance gene under the control of the Oct-4 promoter. G418 selection allowed the isolation of undifferentiated stem cells expressing Oct-4 protein out of human amniotic fluid samples. Our findings confirm the existence of stem cells within amniotic fluid. In addition, the ability to transfect human amniotic fluid cells and to isolate stem-cell marker-positive cells will provide the means to study and manipulate these cells for the purpose of basic and applied research.

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Copy and paste a formatted citation
Spandidos Publications style
Karlmark KR, Freilinger A, Marton E, Rosner M, Lubec G and Hengstschläger M: Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells. Int J Mol Med 16: 987-992, 2005.
APA
Karlmark, K.R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., & Hengstschläger, M. (2005). Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells. International Journal of Molecular Medicine, 16, 987-992. https://doi.org/10.3892/ijmm.16.6.987
MLA
Karlmark, K. R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., Hengstschläger, M."Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells". International Journal of Molecular Medicine 16.6 (2005): 987-992.
Chicago
Karlmark, K. R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., Hengstschläger, M."Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells". International Journal of Molecular Medicine 16, no. 6 (2005): 987-992. https://doi.org/10.3892/ijmm.16.6.987
Copy and paste a formatted citation
x
Spandidos Publications style
Karlmark KR, Freilinger A, Marton E, Rosner M, Lubec G and Hengstschläger M: Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells. Int J Mol Med 16: 987-992, 2005.
APA
Karlmark, K.R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., & Hengstschläger, M. (2005). Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells. International Journal of Molecular Medicine, 16, 987-992. https://doi.org/10.3892/ijmm.16.6.987
MLA
Karlmark, K. R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., Hengstschläger, M."Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells". International Journal of Molecular Medicine 16.6 (2005): 987-992.
Chicago
Karlmark, K. R., Freilinger, A., Marton, E., Rosner, M., Lubec, G., Hengstschläger, M."Activation of ectopic Oct-4 and Rex-1 promoters in human amniotic fluid cells". International Journal of Molecular Medicine 16, no. 6 (2005): 987-992. https://doi.org/10.3892/ijmm.16.6.987
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