Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice

  • Authors:
    • Kazunori Yukawa
    • Tetsuji Tanaka
    • Tao Bai
    • Li Li
    • Yuji Tsubota
    • Kyoko Owada-Makabe
    • Masanobu Maeda
    • Katsuaki Hoshino
    • Shizuo Akira
    • Hiroyuki Iso
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijmm.17.5.869
  • Pages: 869-873
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Abstract

Death-associated protein kinase (DAPK) is a Ca2+/calmodulin-dependent serine/threonine kinase that is thought to mediate apoptosis. DAPK is highly expressed in hippocampal neurons which are essential elements for memory formation. To examine if DAPK is implicated in spatial learning and memory, both wild-type and DAPK-mutant mice were subjected to Morris water maze tests. DAPK-mutant mice were generated by deleting 74 amino acids from the catalytic kinase domain of DAPK, and were used to investigate roles of the DAPK kinase domain in regulating spatial memory. Both mutant and wild-type mice were able to learn the water maze tasks to locate a hidden escape platform. In the first probe test, mutant mice showed a more precise memory for platform position compared to wild-type mice. In the reversal training in which the platform was located opposite from the original position, DAPK-mutant mice exhibited superior spatial learning compared to wild-type mice. DAPK-mutant mice also showed a more precise memory than their wild-type littermates in the probe trial of reversal test. Thus, the present results revealed crucial implications of DAPK in regulating spatial memory in mice.

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May 2006
Volume 17 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yukawa K, Tanaka T, Bai T, Li L, Tsubota Y, Owada-Makabe K, Maeda M, Hoshino K, Akira S, Iso H, Iso H, et al: Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice. Int J Mol Med 17: 869-873, 2006.
APA
Yukawa, K., Tanaka, T., Bai, T., Li, L., Tsubota, Y., Owada-Makabe, K. ... Iso, H. (2006). Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice. International Journal of Molecular Medicine, 17, 869-873. https://doi.org/10.3892/ijmm.17.5.869
MLA
Yukawa, K., Tanaka, T., Bai, T., Li, L., Tsubota, Y., Owada-Makabe, K., Maeda, M., Hoshino, K., Akira, S., Iso, H."Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice". International Journal of Molecular Medicine 17.5 (2006): 869-873.
Chicago
Yukawa, K., Tanaka, T., Bai, T., Li, L., Tsubota, Y., Owada-Makabe, K., Maeda, M., Hoshino, K., Akira, S., Iso, H."Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice". International Journal of Molecular Medicine 17, no. 5 (2006): 869-873. https://doi.org/10.3892/ijmm.17.5.869