Adrenomedullin binding protein-1 is downregulated during polymicrobial sepsis in the rat

  • Authors:
    • Yingjie Cui
    • Youxin Ji
    • Rongqian Wu
    • Mian Zhou
    • Ping Wang
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijmm.17.5.925
  • Pages: 925-929
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Abstract

We have previously shown that administration of adrenomedullin (AM) and AM binding protein-1 (AMBP-1) in combination maintains cardiovascular stability and reduces mortality in a rat model of sepsis. However, it is unknown whether AMBP-1 is reduced under the septic condition and, if so, whether lipopolysaccharide (LPS) plays a role in down-regulating AMBP-1. To determine this, male adult Sprague-Dawley rats were subjected to either polymicrobial sepsis by cecal ligation and puncture (CLP), or endotoxemia by intraperitoneal injection of LPS (15 mg/kg body weight). In an additional group of animals, LPS neutralizing agent polymyxin B (PMB) was given intramuscularly at 0.5 h before and 9 h after CLP. At 20 h after CLP (i.e. the late stage of sepsis) or endotoxemia, hepatic tissue and blood samples were collected. Hepatic AMBP-1 gene expression along with hepatic and plasma AMBP-1 protein levels were measured by RT-PCR and Western blot analysis, respectively. Our results showed that hepatic AMBP-1 gene expression decreased by 65%, hepatic AMBP-1 protein levels decreased by 72%, and plasma levels of AMBP-1 decreased by 59% at 20 h after CLP. Similar results were also seen in the animals receiving LPS injection. Administration of PMB, however, prevented the downregulation of AMBP-1 expression at 20 h after CLP. Thus, AMBP-1 is downregulated in the late phase of sepsis, and LPS plays a critical role in the reduction of AMBP-1.

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May 2006
Volume 17 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cui Y, Ji Y, Wu R, Zhou M and Wang P: Adrenomedullin binding protein-1 is downregulated during polymicrobial sepsis in the rat. Int J Mol Med 17: 925-929, 2006
APA
Cui, Y., Ji, Y., Wu, R., Zhou, M., & Wang, P. (2006). Adrenomedullin binding protein-1 is downregulated during polymicrobial sepsis in the rat. International Journal of Molecular Medicine, 17, 925-929. https://doi.org/10.3892/ijmm.17.5.925
MLA
Cui, Y., Ji, Y., Wu, R., Zhou, M., Wang, P."Adrenomedullin binding protein-1 is downregulated during polymicrobial sepsis in the rat". International Journal of Molecular Medicine 17.5 (2006): 925-929.
Chicago
Cui, Y., Ji, Y., Wu, R., Zhou, M., Wang, P."Adrenomedullin binding protein-1 is downregulated during polymicrobial sepsis in the rat". International Journal of Molecular Medicine 17, no. 5 (2006): 925-929. https://doi.org/10.3892/ijmm.17.5.925