Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro

  • Authors:
    • Marco Galli
    • Caterina Toriani Terenzi
    • Gianluca Falcone
    • Ubaldo Pozzetto
    • Giuseppe Tringali
    • Vincenzo De Santis
    • Mauro Vairano
    • Pierluigi Navarra
    • Giacomo Pozzoli
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijmm.18.1.141
  • Pages: 141-145
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In this study the potential of clinically relevant alumina ceramic and metal wear particles to induce an in vitro inflammatory response was assessed in human monocytes and lymphocytes isolated from healthy donors by measuring pro-staglandin E2 (PGE2) levels and mRNA expression of various pro-inflammatory cytokines. Bacterial lipopolysaccharide (LPS) was used as positive control. LPS significantly increased PGE2 levels in the incubation medium of monocyte cultures after 24 h. Alumina had no effect on PGE2 production, whereas metals induced a concentration-dependent increase in PGE2 release, that was statistically significant at the dose of 0.1 mg/ ml. In lymphocytes, LPS elicited a weak but significant increase in PGE2 release, whereas both alumina and metals did not modify PGE2 amounts at any of the concentrations tested. The gene expression of a number of pro- and anti-inflammatory cytokines was assessed in monocytes and lymphocytes exposed to LPS, 0.1 mg/ml alumina or 0.1 mg/ml metals for 24 h. In monocytes, LPS caused a 2-fold increase in interleukin-1β (IL-1β) mRNA levels. The exposure of monocytes to metals resulted in a selective increase in IL-1β mRNA accumulation (+48% compared to control). By contrast, alumina did not modify IL-1β mRNA levels. None of the test substances elicited any response on purified lymphocyte population. These findings suggest that PGE2 production and IL-1 mRNA expression are a reliable marker to study the pro-inflammatory effects of wear debris in vitro. The lower activity of alumina compared to metals suggests that the former should be pre-ferred in implants for its favorable biological and mechanical behavior.

Related Articles

Journal Cover

July 2006
Volume 18 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Galli M, Toriani Terenzi C, Falcone G, Pozzetto U, Tringali G, De Santis V, Vairano M, Navarra P and Pozzoli G: Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro. Int J Mol Med 18: 141-145, 2006
APA
Galli, M., Toriani Terenzi, C., Falcone, G., Pozzetto, U., Tringali, G., De Santis, V. ... Pozzoli, G. (2006). Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro. International Journal of Molecular Medicine, 18, 141-145. https://doi.org/10.3892/ijmm.18.1.141
MLA
Galli, M., Toriani Terenzi, C., Falcone, G., Pozzetto, U., Tringali, G., De Santis, V., Vairano, M., Navarra, P., Pozzoli, G."Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro". International Journal of Molecular Medicine 18.1 (2006): 141-145.
Chicago
Galli, M., Toriani Terenzi, C., Falcone, G., Pozzetto, U., Tringali, G., De Santis, V., Vairano, M., Navarra, P., Pozzoli, G."Metallic but not ceramic wear particles increase prostaglandin E2 release and interleukin-1β gene expression in human blood monocytes in vitro". International Journal of Molecular Medicine 18, no. 1 (2006): 141-145. https://doi.org/10.3892/ijmm.18.1.141