TGFβ induces transdifferentiation of iBREC to αSMA-expressing cells

  • Authors:
    • Heidrun Deissler
    • Helmut Deissler
    • Gerhard K. Lang
    • Gabriele E. Lang
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.577
  • Pages: 577-582
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Abstract

Transforming growth factor β (TGFβ) both inhibits proliferation of macrovascular endothelial cells and promotes their transdifferentiation to α-smooth-muscle-actin (αSMA)-expressing mesenchymal cells in vitro. Recently, we have confirmed that proliferation of immortalized bovine retinal microvascular endothelial cells (iBREC) is strongly inhibited by TGFβ2. We now demonstrate a complete transition of both parental iBREC and single cell-derived subclones from cobblestone morphology to a ragged appearance as a consequence of incubation for a few days with 10 ng/ml TGFβ1 or TGFβ2. Depending on the type of culture medium, 5-40% of these cells strongly expressed αSMA after approximately 6 days whereas expression of the endothelial cell-specific marker proteins von Willebrand factor and VE Cadherin (CD144) declined. Expression of αSMA, associated with formation of stress fibers, was first detected in single cells and then spread to adjacent cells, and declined slowly after prolonged cultivation in medium without TGFβ2. However, re-constitution of vWF expression was not observed. TGFβ2-induced phenotypic alterations were specific, as they were not caused by treatment of iBREC with VEGF, IGF-1 or bFGF. Induction of αSMA expression but not effects on morphology was strongly inhibited by bFGF, whereas IGF-1 enhanced TGFβ2-induced αSMA expression. These findings may have an important impact on the understanding of development of microvascular complications of diabetes such as diabetic retinopathy.

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October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Deissler H, Deissler H, Lang GK and Lang GE: TGFβ induces transdifferentiation of iBREC to αSMA-expressing cells. Int J Mol Med 18: 577-582, 2006
APA
Deissler, H., Deissler, H., Lang, G.K., & Lang, G.E. (2006). TGFβ induces transdifferentiation of iBREC to αSMA-expressing cells. International Journal of Molecular Medicine, 18, 577-582. https://doi.org/10.3892/ijmm.18.4.577
MLA
Deissler, H., Deissler, H., Lang, G. K., Lang, G. E."TGFβ induces transdifferentiation of iBREC to αSMA-expressing cells". International Journal of Molecular Medicine 18.4 (2006): 577-582.
Chicago
Deissler, H., Deissler, H., Lang, G. K., Lang, G. E."TGFβ induces transdifferentiation of iBREC to αSMA-expressing cells". International Journal of Molecular Medicine 18, no. 4 (2006): 577-582. https://doi.org/10.3892/ijmm.18.4.577