Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy

  • Authors:
    • Masahiko Kaito
    • Shozo Watanabe
    • Hideaki Tanaka
    • Naoki Fujita
    • Masayoshi Konishi
    • Motoh Iwasa
    • Yoshinao Kobayashi
    • Esteban Cesar Gabazza
    • Yukihiko Adachi
    • Kyoko Tsukiyama-Kohara
    • Michinori Kohara
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.673
  • Pages: 673-678
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

It is known that hepatitis C virus (HCV) particles are spherical, 55-65 nm particles with fine surface projections of about 6 nm in length and with a 30-35 nm inner core. We have reported that free HCV particles labeled with gold particles specific to the HCV E1 glycoprotein are located in 1.14-1.16 g/ml fractions from plasma samples with high HCV RNA titers after sucrose density gradient centrifugation. However, the morphology of the HCV E2 glycoprotein on the virion has not yet been elucidated. To visualize HCV E2 localization on the virion, we used the same plasma samples where HCV particles were clearly shown. An indirect immunogold electron microscopic study was carried out using monoclonal and polyclonal anti-HCV E2 antibodies. HCV-like particles specifically reacted with the anti-HCV E2 antibodies. Moreover, to evaluate the localization of the HCV E1 and E2 glycoproteins on the virion surface, an immunogold electron microscopic study using double labeling with anti-HCV E1 antibodies and anti-HCV E2 antibodies was also performed. These particles also specifically reacted with both anti-E1 and E2 antibodies. This is the first report showing the presence of both HCV E1 and E2 glycoproteins on HCV virion surface in human plasma samples.

Related Articles

Journal Cover

October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kaito M, Watanabe S, Tanaka H, Fujita N, Konishi M, Iwasa M, Kobayashi Y, Gabazza EC, Adachi Y, Tsukiyama-Kohara K, Tsukiyama-Kohara K, et al: Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. Int J Mol Med 18: 673-678, 2006.
APA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M. ... Kohara, M. (2006). Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. International Journal of Molecular Medicine, 18, 673-678. https://doi.org/10.3892/ijmm.18.4.673
MLA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18.4 (2006): 673-678.
Chicago
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18, no. 4 (2006): 673-678. https://doi.org/10.3892/ijmm.18.4.673