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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2006 Volume 18 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy

  • Authors:
    • Masahiko Kaito
    • Shozo Watanabe
    • Hideaki Tanaka
    • Naoki Fujita
    • Masayoshi Konishi
    • Motoh Iwasa
    • Yoshinao Kobayashi
    • Esteban Cesar Gabazza
    • Yukihiko Adachi
    • Kyoko Tsukiyama-Kohara
    • Michinori Kohara
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Hepatology, Division of Clinical Medicine and Biomedical Science, Institute of Medical Science, Mie University Graduate School of Medicine, Mie 514-8507, Japan. kaitoma@clin.medic.mie-u.ac.jp
  • Pages: 673-678
    |
    Published online on: October 1, 2006
       https://doi.org/10.3892/ijmm.18.4.673
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Abstract

It is known that hepatitis C virus (HCV) particles are spherical, 55-65 nm particles with fine surface projections of about 6 nm in length and with a 30-35 nm inner core. We have reported that free HCV particles labeled with gold particles specific to the HCV E1 glycoprotein are located in 1.14-1.16 g/ml fractions from plasma samples with high HCV RNA titers after sucrose density gradient centrifugation. However, the morphology of the HCV E2 glycoprotein on the virion has not yet been elucidated. To visualize HCV E2 localization on the virion, we used the same plasma samples where HCV particles were clearly shown. An indirect immunogold electron microscopic study was carried out using monoclonal and polyclonal anti-HCV E2 antibodies. HCV-like particles specifically reacted with the anti-HCV E2 antibodies. Moreover, to evaluate the localization of the HCV E1 and E2 glycoproteins on the virion surface, an immunogold electron microscopic study using double labeling with anti-HCV E1 antibodies and anti-HCV E2 antibodies was also performed. These particles also specifically reacted with both anti-E1 and E2 antibodies. This is the first report showing the presence of both HCV E1 and E2 glycoproteins on HCV virion surface in human plasma samples.

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Copy and paste a formatted citation
Spandidos Publications style
Kaito M, Watanabe S, Tanaka H, Fujita N, Konishi M, Iwasa M, Kobayashi Y, Gabazza EC, Adachi Y, Tsukiyama-Kohara K, Tsukiyama-Kohara K, et al: Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. Int J Mol Med 18: 673-678, 2006.
APA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M. ... Kohara, M. (2006). Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. International Journal of Molecular Medicine, 18, 673-678. https://doi.org/10.3892/ijmm.18.4.673
MLA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18.4 (2006): 673-678.
Chicago
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18, no. 4 (2006): 673-678. https://doi.org/10.3892/ijmm.18.4.673
Copy and paste a formatted citation
x
Spandidos Publications style
Kaito M, Watanabe S, Tanaka H, Fujita N, Konishi M, Iwasa M, Kobayashi Y, Gabazza EC, Adachi Y, Tsukiyama-Kohara K, Tsukiyama-Kohara K, et al: Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. Int J Mol Med 18: 673-678, 2006.
APA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M. ... Kohara, M. (2006). Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. International Journal of Molecular Medicine, 18, 673-678. https://doi.org/10.3892/ijmm.18.4.673
MLA
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18.4 (2006): 673-678.
Chicago
Kaito, M., Watanabe, S., Tanaka, H., Fujita, N., Konishi, M., Iwasa, M., Kobayashi, Y., Gabazza, E. C., Adachi, Y., Tsukiyama-Kohara, K., Kohara, M."Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy". International Journal of Molecular Medicine 18, no. 4 (2006): 673-678. https://doi.org/10.3892/ijmm.18.4.673
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