Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3-depleted rats

  • Authors:
    • S. Peltier
    • W. J. Malaisse
    • L. Portois
    • L. Demaison
    • V. Novel-Chate
    • J. M. Chardigny
    • J. L. Sebedio
    • Y. A. Carpentier
    • X. M. Leverve
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijmm.18.4.741
  • Pages: 741-749
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Abstract

A novel i.v. lipid preparation (MCT:FO) containing 80% medium chain-triacylglycerols and 20% fish oil was recently developed to rapidly replenish cell membrane phospholipids with omega 3 (n-3) polyunsaturated fatty acids (PUFA). In regard of this property, we investigated the effect of a single i.v. administration of MCT:FO on the recovery of cardiac function after ischemia in control and n-3-depleted rats. Results were compared with those obtained either with a control preparation, where FO was replaced by triolein (MCT:OO), or with saline. Saline (1 ml) or lipid preparation (also 1 ml) was injected as a bolus via the left saphenous vein. After 60 min the heart was removed and perfused for 20 min in normoxic conditions according to Langendorff. Thereafter, the heart was subjected to a 20 min zero-flow normothermic ischemia, followed by 40 min reperfusion. Cardiac mechanical and metabolic functions were monitored. In control rats, the previous administration of a lipid preparation (MCT:FO or MCT:OO) versus saline improved cardiac function during aerobic reperfusion post-ischemia. N-3-depleted rats showed decreased basal cardiac function and impaired recovery following ischemia. However, the bolus injection of MCT:FO opposed the deleterious effect of long-term n-3-deficiency and, in this respect, was superior to MCT:OO over the first 20 min of reperfusion. This novel approach to rapidly correct n-3 PUFA-deficiency might be clinically relevant and offer interesting perspectives in the management of acute ischemic accidents.

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October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Peltier S, Malaisse WJ, Portois L, Demaison L, Novel-Chate V, Chardigny JM, Sebedio JL, Carpentier YA and Leverve XM: Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3-depleted rats. Int J Mol Med 18: 741-749, 2006.
APA
Peltier, S., Malaisse, W.J., Portois, L., Demaison, L., Novel-Chate, V., Chardigny, J.M. ... Leverve, X.M. (2006). Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3-depleted rats. International Journal of Molecular Medicine, 18, 741-749. https://doi.org/10.3892/ijmm.18.4.741
MLA
Peltier, S., Malaisse, W. J., Portois, L., Demaison, L., Novel-Chate, V., Chardigny, J. M., Sebedio, J. L., Carpentier, Y. A., Leverve, X. M."Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3-depleted rats". International Journal of Molecular Medicine 18.4 (2006): 741-749.
Chicago
Peltier, S., Malaisse, W. J., Portois, L., Demaison, L., Novel-Chate, V., Chardigny, J. M., Sebedio, J. L., Carpentier, Y. A., Leverve, X. M."Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3-depleted rats". International Journal of Molecular Medicine 18, no. 4 (2006): 741-749. https://doi.org/10.3892/ijmm.18.4.741