Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line

  • Authors:
    • Tobias Müller
    • Daniel Myrtek
    • Hannes Bayer
    • Stephan Sorichter
    • Katrin Schneider
    • Gernot Zissel
    • Johannes Norgauer
    • Marco Idzko
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijmm.18.5.925
  • Pages: 925-931
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Histamine is a well-known mediator eliciting a broad range of responses in different cell types. Four different subtypes of G protein-coupled histamine receptors (H1-H4) have been cloned and pharmacologically characterized. However, involvement of the different histamine receptor subtypes in immunomodulatory functions of bronchial epithelium has only been investigated marginally. The expression and function of histamine receptor subtypes on the human bronchial epithelial cell line BEAS-2B was analyzed by PCR, intracellular Ca++-measurements and ELISA. We show mRNA expression of the histamine receptor subtypes H1, H2, and H3, but not H4 in the human bronchial epithelial cell line BEAS-2B. Using intracellular Ca++-measurements, we demonstrated functional expression of the H1 and H3 receptors. To characterize the biological properties of histamine in airway epithelial biology, we also investigated its effects on cytokine secretion by BEAS-2B cells. Thereby, we were able to show up-regulation of the proinflammatory mediators IL-6 and CXCL8/ IL-8 via activation of the H1, H2 and H3 receptor subtypes. The Th1 cytokines CXCL9/MIG and CXCL10/IP-10 and the chemokine CCL5/RANTES were regulated in a distinct manner: Whereas histamine inhibited the IFN-γ/TNF-α-induced secretion of MIG via the histamine receptor subtypes H1, H2, and H3, the histamine-induced suppression of RANTES was due to activation of the H2 and H3 receptors, while reduction of cytokine-triggered IP-10 secretion was mediated only by triggering the H2 receptor. In summary our data provide evidence that histamine released during allergic lung diseases exerts regulatory influence on airway epithelial cells.

Related Articles

Journal Cover

November 2006
Volume 18 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Müller T, Myrtek D, Bayer H, Sorichter S, Schneider K, Zissel G, Norgauer J and Idzko M: Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line. Int J Mol Med 18: 925-931, 2006.
APA
Müller, T., Myrtek, D., Bayer, H., Sorichter, S., Schneider, K., Zissel, G. ... Idzko, M. (2006). Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line. International Journal of Molecular Medicine, 18, 925-931. https://doi.org/10.3892/ijmm.18.5.925
MLA
Müller, T., Myrtek, D., Bayer, H., Sorichter, S., Schneider, K., Zissel, G., Norgauer, J., Idzko, M."Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line". International Journal of Molecular Medicine 18.5 (2006): 925-931.
Chicago
Müller, T., Myrtek, D., Bayer, H., Sorichter, S., Schneider, K., Zissel, G., Norgauer, J., Idzko, M."Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line". International Journal of Molecular Medicine 18, no. 5 (2006): 925-931. https://doi.org/10.3892/ijmm.18.5.925