Antiangiogenic activity of a concentrated effective microorganism fermentation extract

  • Authors:
    • Chung Hin Chui
    • Roberto Gambari
    • Fung Yi Lau
    • Desmond Kwok Po Hau
    • Raymond Siu Ming Wong
    • Gregory Yin Ming Cheng
    • Stanton Hon Lung Kok
    • Teruo Higa
    • Bin Ke
    • Albert Sun Chi Chan
    • David Wan Fun Fong
    • Johnny Cheuk On Tang
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijmm.18.5.975
  • Pages: 975-979
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Abstract

We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demonstrated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.

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November 2006
Volume 18 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chui CH, Gambari R, Lau FY, Hau DK, Wong RS, Cheng GY, Kok SH, Higa T, Ke B, Chan AS, Chan AS, et al: Antiangiogenic activity of a concentrated effective microorganism fermentation extract. Int J Mol Med 18: 975-979, 2006.
APA
Chui, C.H., Gambari, R., Lau, F.Y., Hau, D.K., Wong, R.S., Cheng, G.Y. ... Tang, J.C. (2006). Antiangiogenic activity of a concentrated effective microorganism fermentation extract. International Journal of Molecular Medicine, 18, 975-979. https://doi.org/10.3892/ijmm.18.5.975
MLA
Chui, C. H., Gambari, R., Lau, F. Y., Hau, D. K., Wong, R. S., Cheng, G. Y., Kok, S. H., Higa, T., Ke, B., Chan, A. S., Fong, D. W., Tang, J. C."Antiangiogenic activity of a concentrated effective microorganism fermentation extract". International Journal of Molecular Medicine 18.5 (2006): 975-979.
Chicago
Chui, C. H., Gambari, R., Lau, F. Y., Hau, D. K., Wong, R. S., Cheng, G. Y., Kok, S. H., Higa, T., Ke, B., Chan, A. S., Fong, D. W., Tang, J. C."Antiangiogenic activity of a concentrated effective microorganism fermentation extract". International Journal of Molecular Medicine 18, no. 5 (2006): 975-979. https://doi.org/10.3892/ijmm.18.5.975