Adenoviral transduction efficiency partly correlates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells.

  • Authors:
    • S Tatebe
    • T Matsuura
    • K Endo
    • R Doi
    • A Goto
    • K Sato
    • H Ito
  • View Affiliations

  • Published online on: July 1, 1998     https://doi.org/10.3892/ijmm.2.1.61
  • Pages: 61-65
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Abstract

Adenovirus has attracted much attention as a vector for gene therapy. Integrin alphavbeta3 and alphavbeta5 mediate adenovirus internalization into cells. We examined adenoviral transduction efficiency and expression of integrin alphavbeta3 alphavbeta5 in 9 human gastric carcinoma cell lines. The percentage of -gal-positive cells was more than 85% 3 days after infection with recombinant adenovirus carrying the bacterial LacZ gene (AxCALacZ) at a dose of 25 MOI in 7 cell lines and the transduction efficiency was 32 and 42% in HSC-39 and MKN-28 cells, respectively. Adenoviral transduction efficiency did not correlate with the histological cell types. Flow cytometric analysis revealed relatively high expression of integrin alphavbeta5 in MKN-28 and OCUM-2M cells, followed by MKN-1, MKN-7, MKN-45, MKN-74, TMK-1 and KATO-III cells. HSC-39 cells minimally expressed integrin alphavbeta5. On the other hand, integrin alphavbeta3 was expressed only in MKN-1 cells. These results suggest that the adenovirus vector might be useful for gene transduction into human gastric carcinoma cells and the transduction efficiency partly correlates with the expression levels of integrin alphavbeta5 but not integrin alphavbeta3.

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Jul 1998
Volume 2 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tatebe S, Matsuura T, Endo K, Doi R, Goto A, Sato K and Ito H: Adenoviral transduction efficiency partly correlates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells.. Int J Mol Med 2: 61-65, 1998
APA
Tatebe, S., Matsuura, T., Endo, K., Doi, R., Goto, A., Sato, K., & Ito, H. (1998). Adenoviral transduction efficiency partly correlates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells.. International Journal of Molecular Medicine, 2, 61-65. https://doi.org/10.3892/ijmm.2.1.61
MLA
Tatebe, S., Matsuura, T., Endo, K., Doi, R., Goto, A., Sato, K., Ito, H."Adenoviral transduction efficiency partly correlates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells.". International Journal of Molecular Medicine 2.1 (1998): 61-65.
Chicago
Tatebe, S., Matsuura, T., Endo, K., Doi, R., Goto, A., Sato, K., Ito, H."Adenoviral transduction efficiency partly correlates with expression levels of integrin alphavbeta5, but not alphavbeta3 in human gastric carcinoma cells.". International Journal of Molecular Medicine 2, no. 1 (1998): 61-65. https://doi.org/10.3892/ijmm.2.1.61