Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors

  • Authors:
    • Agnieszka Ziolkowska
    • Marcin Rucinski
    • Cinzia Tortorella
    • Marianna Tyczewska
    • Gastone G. Nussdorfer
    • Ludwik K. Malendowicz
  • View Affiliations

  • Published online on: December 1, 2007     https://doi.org/10.3892/ijmm.20.6.779
  • Pages: 779-782
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Orexins A and B are hypothalamic peptides which are derived from the proteolytic cleavage of prepro-orexin and act via two subtypes of receptors, named OX1-R (that almost exclusively binds orexin-A) and OX2-R (nonselective for both orexins). Several lines of evidence show that other neuropeptides, which like orexins are involved in the central control of energy homeostasis (e.g. leptin and ghrelin), may play a role in the regulation of bone metabolism, acting via autocrine-paracrine or endocrine routes. Therefore, we studied by reverse transcription-polymerase chain reaction (RT-PCR) the expression of the orexin system in rat calvarial osteoblast-like (ROB) cells, whose osteoblastic lineage was immunocytochemically demonstrated by their osteonectin and collagen-1α content at day 14 of culture. Conventional PCR detected the mRNA expression of OX1-R, but not OX2-R and prepro-orexin in ROB cells at days 2, 7 and 21 of culture. Semiquantitative real time-PCR evidenced a gradual down-regulation of OX1-R mRNA in relation to the duration of culture. This novel finding suggests that rat osteoblasts could be a target for circulating orexin-A, especially during their early stages of differentiation into mature osteoblasts.

Related Articles

Journal Cover

December 2007
Volume 20 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ziolkowska A, Rucinski M, Tortorella C, Tyczewska M, Nussdorfer GG and Malendowicz LK: Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors. Int J Mol Med 20: 779-782, 2007.
APA
Ziolkowska, A., Rucinski, M., Tortorella, C., Tyczewska, M., Nussdorfer, G.G., & Malendowicz, L.K. (2007). Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors. International Journal of Molecular Medicine, 20, 779-782. https://doi.org/10.3892/ijmm.20.6.779
MLA
Ziolkowska, A., Rucinski, M., Tortorella, C., Tyczewska, M., Nussdorfer, G. G., Malendowicz, L. K."Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors". International Journal of Molecular Medicine 20.6 (2007): 779-782.
Chicago
Ziolkowska, A., Rucinski, M., Tortorella, C., Tyczewska, M., Nussdorfer, G. G., Malendowicz, L. K."Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors". International Journal of Molecular Medicine 20, no. 6 (2007): 779-782. https://doi.org/10.3892/ijmm.20.6.779