Ultraviolet A exposure induces reversible disruption of gap junction intercellular communication in lens epithelial cells

  • Authors:
    • Dayang Wu
    • Jiangyue Zhao
    • Di Wu
    • Jinsong Zhang
  • View Affiliations

  • Published online on: March 31, 2011     https://doi.org/10.3892/ijmm.2011.665
  • Pages: 239-245
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Abstract

Gap junction intercellular communication (GJIC) is essential for the proper function of many organs including the lens. Disruption of GJIC can cause lens metabolic disorder and can induce cataracts. The purpose of this study was to investigate the signal transduction pathways involved in GJIC disruption following ultraviolet A (UVA) exposure in lens epithelial cells. Following exposure of human lens epithelial cells to UVA, connexin 43 (Cx43), the main component of gap junctions, was down-regulated at both the mRNA and protein levels. Furthermore, we observed that UVA exposure can increase protein kinase C activity and stimulate reactive oxygen species generation and lipid peroxidation. Using scrape load dye transfer technique, we found that the GJIC is compromised by UVA exposure. In addition, we demonstrated that UVA-induced modulation of GJIC was associated with p38 mitogen-activated protein kinase activation. More importantly, at non-lethal doses (10 J/cm2), the UVA-induced GJIC disruption and the consequent alterations were reversible. Collectively, our data revealed a new signaling pathway in GJIC disruption following UVA exposure, suggesting that UVA-compromised gap junction activity may sensitize human lens to photoaging and cataract formation.

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August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wu D, Zhao J, Wu D and Zhang J: Ultraviolet A exposure induces reversible disruption of gap junction intercellular communication in lens epithelial cells. Int J Mol Med 28: 239-245, 2011
APA
Wu, D., Zhao, J., Wu, D., & Zhang, J. (2011). Ultraviolet A exposure induces reversible disruption of gap junction intercellular communication in lens epithelial cells. International Journal of Molecular Medicine, 28, 239-245. https://doi.org/10.3892/ijmm.2011.665
MLA
Wu, D., Zhao, J., Wu, D., Zhang, J."Ultraviolet A exposure induces reversible disruption of gap junction intercellular communication in lens epithelial cells". International Journal of Molecular Medicine 28.2 (2011): 239-245.
Chicago
Wu, D., Zhao, J., Wu, D., Zhang, J."Ultraviolet A exposure induces reversible disruption of gap junction intercellular communication in lens epithelial cells". International Journal of Molecular Medicine 28, no. 2 (2011): 239-245. https://doi.org/10.3892/ijmm.2011.665