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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February 2012 Volume 29 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii

  • Authors:
    • Jae Yeong Park
    • Jung Eun Park
    • Jong Woo Park
    • Seong Myeong Yoon
    • Jung Sup Lee

  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, College of Natural Sciences, Chosun University, Gwangju 501-759, Republic of Korea, Department of Biotechnology, College of Natural Sciences, Chosun University, 375 Seosuk-dong Dong-gu, Gwangju 501-759, Republic of Korea
  • Pages: 263-268
    |
    Published online on: October 13, 2011
       https://doi.org/10.3892/ijmm.2011.813
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Abstract

A novel extracellular alkaline serine protease secreted by Vibrio metschnikovii (V. metschnikovii) ATCC700040 cells was purified by three chromatographic steps and characterized in terms of enzymatic kinetics and substrate specificity. The purified enzyme (named AKP-Vm) was composed of a single polypeptide with an apparent molecular weight of 50 kDa on 12% SDS-polyacrylamide gel in the presence of CuCl2. The optimal temperature and the pH for the enzyme were found to be 37˚C and 9.5, respectively. However, the enzyme activity was inhibited by inhibitors such as PMSF and aprotinin. AKP-Vm could hydrolyze a peptide bond at the carboxyl side of the arginine residue, as revealed by its amidolytic activity toward a chromogenic substrate, Boc-Val-Pro-Arg-pNA. The kinetic parameters of the enzyme were as follows: KM=0.91 mM, kcat=0.8 sec-1 and kcat/KM=0.88 mM-1sec-1. AKP-Vm protease could cleave various blood coagulation-associated proteins, including fibrinogen, prothrombin and thrombin. In particular, the enzyme showed powerful fibrinogenolytic and fibrinolytic activities, as it could cleave all major chains of fibrinogen and also digest cross-linked fibrin. The results obtained suggest that AKP-Vm is a novel alkaline serine protease that can actively cleave fibrinogen and cross-linked fibrin.

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Copy and paste a formatted citation
Spandidos Publications style
Park JY, Park JE, Park JW, Yoon SM and Lee JS: Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii. Int J Mol Med 29: 263-268, 2012.
APA
Park, J.Y., Park, J.E., Park, J.W., Yoon, S.M., & Lee, J.S. (2012). Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii. International Journal of Molecular Medicine, 29, 263-268. https://doi.org/10.3892/ijmm.2011.813
MLA
Park, J. Y., Park, J. E., Park, J. W., Yoon, S. M., Lee, J. S."Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii". International Journal of Molecular Medicine 29.2 (2012): 263-268.
Chicago
Park, J. Y., Park, J. E., Park, J. W., Yoon, S. M., Lee, J. S."Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii". International Journal of Molecular Medicine 29, no. 2 (2012): 263-268. https://doi.org/10.3892/ijmm.2011.813
Copy and paste a formatted citation
x
Spandidos Publications style
Park JY, Park JE, Park JW, Yoon SM and Lee JS: Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii. Int J Mol Med 29: 263-268, 2012.
APA
Park, J.Y., Park, J.E., Park, J.W., Yoon, S.M., & Lee, J.S. (2012). Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii. International Journal of Molecular Medicine, 29, 263-268. https://doi.org/10.3892/ijmm.2011.813
MLA
Park, J. Y., Park, J. E., Park, J. W., Yoon, S. M., Lee, J. S."Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii". International Journal of Molecular Medicine 29.2 (2012): 263-268.
Chicago
Park, J. Y., Park, J. E., Park, J. W., Yoon, S. M., Lee, J. S."Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii". International Journal of Molecular Medicine 29, no. 2 (2012): 263-268. https://doi.org/10.3892/ijmm.2011.813
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