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Clobetasol down-regulates SLPI expression in U937 monocytoid cells

  • Authors:
    • Naoko Okumura
    • Hitomi Yoshida
    • Yasuko Kitagishi
    • Yuri Nishimura
    • Satoru Matsuda

  • View Affiliations

  • Published online on: November 7, 2011     https://doi.org/10.3892/ijmm.2011.825
  • Pages: 324-326
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

In order to investigate how glucocorticoids affect the expression of secretory leukocyte peptidase inhibitor (SLPI), which is overexpressed in a variety of cancers, clobetasol was added to cell culture medium of U937 cells and the SLPI mRNA levels were examined. The in vitro effect of the treatment on SLPI expression was detected by reverse transcriptase-polymerase chain reaction. Clobetasol treatment of U937 cells induced an up- and down-regulation of SLPI expression in a dose-dependent manner. Western blotting confirmed the down-regulation of SLPI protein expression. We hypothesized a loop formation in the SLPI genome domain, in which the glucocorticoid receptor regulates bi-directional transcriptional activity.

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Journal Cover

February 2012
Volume 29 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Okumura N, Yoshida H, Kitagishi Y, Nishimura Y and Matsuda S: Clobetasol down-regulates SLPI expression in U937 monocytoid cells. Int J Mol Med 29: 324-326, 2012
APA
Okumura, N., Yoshida, H., Kitagishi, Y., Nishimura, Y., & Matsuda, S. (2012). Clobetasol down-regulates SLPI expression in U937 monocytoid cells. International Journal of Molecular Medicine, 29, 324-326. https://doi.org/10.3892/ijmm.2011.825
MLA
Okumura, N., Yoshida, H., Kitagishi, Y., Nishimura, Y., Matsuda, S."Clobetasol down-regulates SLPI expression in U937 monocytoid cells". International Journal of Molecular Medicine 29.2 (2012): 324-326.
Chicago
Okumura, N., Yoshida, H., Kitagishi, Y., Nishimura, Y., Matsuda, S."Clobetasol down-regulates SLPI expression in U937 monocytoid cells". International Journal of Molecular Medicine 29, no. 2 (2012): 324-326. https://doi.org/10.3892/ijmm.2011.825