Inhibition airway remodeling and transforming growth factor-β1/Smad signaling pathway by astragalus extract in asthmatic mice Retraction in /10.3892/ijmm.2023.5290

Qu,Zheng-Hai; Yang,Zhao-Chuan; Chen,Lei; Lv,Zhi-Dong; Yi,Ming-Ji; Ran,Ni

doi:10.3892/ijmm.2011.868

Inhibition airway remodeling and transforming growth factor-β1/Smad signaling pathway by astragalus extract in asthmatic mice

Retraction in: /10.3892/ijmm.2023.5290

Authors:
- Zheng-Hai Qu
- Zhao-Chuan Yang
- Lei Chen
- Zhi-Dong Lv
- Ming-Ji Yi
- Ni Ran
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Affiliations: Department of Pediatrics, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, P.R. China
Published online on: December 23, 2011 https://doi.org/10.3892/ijmm.2011.868
Pages: 564-568

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Abstract

Airway remodeling is characterized by airway wall thickening, subepithelial ﬁbrosis, increased smooth muscle mass, angiogenesis and increased mucous glands, which can lead to a chronic and obstinate asthma with pulmonary function depression. In the present study, we investigated whether the astragalus extract inhibits airway remodeling in a mouse asthma model and observed the effects of astragalus extract on the transforming growth factor-β1 (TGF-β1)/Smad signaling pathway in ovalbumin-sensitized mice. Mice were sensitized and challenged by ovalbumin to establish a model of asthma. Treatments included the astragalus extract and budesonide. Lung tissues were obtained for hematoxylin and eosin staining and Periodic acid-Schiff staining after the ﬁnal ovalbumin challenge. Levels of TGF-β1 were assessed by immunohistology and ELISA, levels of TGF-β1 mRNA were measured by RT-PCR, and levels of P-Smad2/3 and T-Smad2/3 were assessed by western blotting. Astragalus extract and budesonide reduced allergen-induced increases in the thickness of bronchial airway and mucous gland hypertrophy, goblet cell hyperplasia and collagen deposition. Levels of lung TGF-β1, TGF-β1 mRNA and P-Smad2/3 were signiﬁcantly reduced in mice treated with astragalus extract and budesonide. Astragalus extract improved asthma airway remodeling by inhibiting the expression of the TGF-β1/Smad signaling pathway, and may be a potential drug for the treatment of patients with a severe asthma airway.

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1	L Di GiampaoloC QuecchiaC SchiavoneE CavallucciA RenzettiM BragaM Di GioacchinoEnvironmental pollution and asthmaInt J Immunopathol Pharmacol24Suppl 1S31S382011
2	WX ZhangCC LiAirway remodeling: a potential therapeutic target in asthmaWorld J Pediatr7124128201110.1007/s12519-011-0264-x21574028
3	M CazzolaMG MateraInhibiting or blocking LIGHT, a TNF superfamily member, for treating airway remodelingExpert Rev Respir Med5623625201110.1586/ers.11.6521955232
4	M HassanT JoPA RisseB TolloczkoC LemièreR OlivensteinQ HamidJG MartinAirway smooth muscle remodeling is a dynamic process in severe long-standing asthmaJ Allergy Clin Immunol12510371045201010.1016/j.jaci.2010.02.03120451038
5	D NaFN LiuZF MiaoZM DuHM XuAstragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosisWorld J Gastroenterol15570577200910.3748/wjg.15.57019195058
6	L ZhangY YangY WangX GaoAstragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injuryPharmazie661441502011
7	GX GaoQM LiHH ShenEffect of Astragali-Cordyceps Mixtura on TGF-beta/Smad signal pathway in the lung of asthma airway remodelingJ Ethnopharmacol1256874200910.1016/j.jep.2009.06.01219549562
8	Y LinB WangXQ LuoClinical study of astragalus’s preventing the recurrence of asthma in childrenZhongguo Zhong Xi Yi Jie He Za Zhi31109010922011(In Chinese)
9	YY WanRA FlavellRegulatory T cells, transforming growth factor-beta, and immune suppressionProc Am Thorac Soc4271276200710.1513/pats.200701-020AW17607012
10	AJ SandfordAsthma susceptibility: the role of transforming growth factor beta1Respirology15583584201010.1111/j.1440-1843.2010.01760.x20546538
11	Y BosséJ StankovaM Rola-PleszczynskiTransforming growth factor-beta1 in asthmatic airway smooth muscle enlargement: is fibroblast growth factor-2 required?Clin Exp Allergy40710724201020447083
12	M ChenZ LvS JiangThe effects of triptolide on airway remodeling and transforming growth factor-1/Smad signaling pathway in ovalbumin-sensitized miceImmunology132376384201110.1111/j.1365-2567.2010.03392.x21214541
13	SE BottomsJE HowellAK ReinhardtIC EvansRJ McAnultyTGF-β isoform specific regulation of airway inflammation and remodeling in a murine model of asthmaPLoS One5e96742010
14	ZD LvD NaXY MaC ZhaoWJ ZhaoHM XuHuman peritoneal mesothelial cell transformation into myofibroblasts in response to TGF-β1 in vitroInt J Mol Med27187193201121152863
15	HY LanDiverse Roles of TGF-β/Smads in renal fibrosis and inflammationInt J Biol Sci7105610672011
16	SG RoyceC LimRC MuljadiML TangTrefoil factor 2 regulates airway remodeling in animal models of asthmaJ Asthma48653659201110.3109/02770903.2011.59990621793772
17	M LiW WangJ XueY GuS LinMeta-analysis of the clinical value of Astragalus membranaceus in diabetic nephropathyJ Ethnopharmacol133412419201110.1016/j.jep.2010.10.01220951192
18	J XueY XuZ ZhangG ShenG ZengThe effect of astragapoly-saccharide on the lymphocyte proliferation and airway inflammation in sensitized miceJ Tongji Med Univ192022199910.1007/BF0289558712840868
19	A de Diego DamiáJM Vega ChicoteTherapeutic approach to the distal airways in asthmaArch Bronconeumol47Suppl 2S27312011(In Spanish)
20	JY ChoA PhamP RosenthalM MillerT DohertyDH BroideChronic OVA allergen challenged TNF p55/p75 receptor deficient mice have reduced airway remodelingInt Immunopharmacol1110381044201110.1016/j.intimp.2011.02.02421382533
21	M GhaneiNA GhalejooghiMR NouraniAA HarandiAA FooladiEffect of TGFβ1 and TIMP2 on disease activity in asthma and COPDIran J Allergy Asthma Immunol979862010
22	W ManuyakornW KamchaisatianK AtamasirikulC SasisakulpornC DirekwattanachaiS BenjaponpitakSerum TGF-beta1 in atopic asthmaAsian Pac J Allergy Immunol26185189200819317336
23	R KowalewskiA MalkowskiK SobolewskiM GackoEvaluation of transforming growth factor-beta signaling pathway in the wall of normal and varicose veinsPathobiology7716201010.1159/00027294820185961
24	KD KiSY TongCY HuhJM LeeSK LeeSG ChiExpression and mutational analysis of TGF-beta/Smads signaling in human cervical cancersJ Gynecol Oncol20117121200910.3802/jgo.2009.20.2.11719590724