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Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy

  • Authors:
    • Steven Carberry
    • Margit Zweyer
    • Dieter Swandulla
    • Kay Ohlendieck
  • View Affiliations / Copyright

    Affiliations: Department of Biology, National University of Ireland, Maynooth, Kildare, Republic of Ireland, Department of Physiology II, University of Bonn, D-53115 Bonn, Germany
    Copyright: © Carberry et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 229-234
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    Published online on: May 18, 2012
       https://doi.org/10.3892/ijmm.2012.1006
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Abstract

Duchenne muscular dystrophy is a lethal genetic disease of childhood caused by primary abnormalities in the gene coding for the membrane cytoskeletal protein dystrophin. The mdx mouse is an established animal model of various aspects of X-linked muscular dystrophy and is widely used for studying fundamental mechanisms of dystrophinopathy and testing novel therapeutic approaches to treat one of the most frequent gender-specific diseases in humans. In order to determine global changes in the muscle proteome with the progressive deterioration of mdx tissue with age, we have characterized diaphragm muscle from mdx mice at three ages (8-weeks, 12-months and 22-months) using mass spectrometry-based proteomics. Altered expression levels in diaphragm of 8-week vs. 22-month mice were shown to occur in 11 muscle-associated proteins. Aging in the mdx diaphragm seems to be associated with a drastic increase in the extracellular matrix proteins, collagen and dermatopontin, the molecular chaperone αB-crystallin, and the intermediate filament protein vimentin, suggesting increased accumulation of connective tissue, an enhanced cellular stress response and compensatory stabilization of the weakened membrane cytoskeleton. These proteomic findings establish the aged mdx diaphragm as an excellent model system for studying secondary effects of dystrophin deficiency in skeletal muscle tissue.
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Spandidos Publications style
Carberry S, Zweyer M, Swandulla D and Ohlendieck K: Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy. Int J Mol Med 30: 229-234, 2012.
APA
Carberry, S., Zweyer, M., Swandulla, D., & Ohlendieck, K. (2012). Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy. International Journal of Molecular Medicine, 30, 229-234. https://doi.org/10.3892/ijmm.2012.1006
MLA
Carberry, S., Zweyer, M., Swandulla, D., Ohlendieck, K."Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy". International Journal of Molecular Medicine 30.2 (2012): 229-234.
Chicago
Carberry, S., Zweyer, M., Swandulla, D., Ohlendieck, K."Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy". International Journal of Molecular Medicine 30, no. 2 (2012): 229-234. https://doi.org/10.3892/ijmm.2012.1006
Copy and paste a formatted citation
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Spandidos Publications style
Carberry S, Zweyer M, Swandulla D and Ohlendieck K: Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy. Int J Mol Med 30: 229-234, 2012.
APA
Carberry, S., Zweyer, M., Swandulla, D., & Ohlendieck, K. (2012). Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy. International Journal of Molecular Medicine, 30, 229-234. https://doi.org/10.3892/ijmm.2012.1006
MLA
Carberry, S., Zweyer, M., Swandulla, D., Ohlendieck, K."Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy". International Journal of Molecular Medicine 30.2 (2012): 229-234.
Chicago
Carberry, S., Zweyer, M., Swandulla, D., Ohlendieck, K."Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophy". International Journal of Molecular Medicine 30, no. 2 (2012): 229-234. https://doi.org/10.3892/ijmm.2012.1006
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