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International Journal of Molecular Medicine
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August 2012 Volume 30 Issue 2

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Article

Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients

  • Authors:
    • Miguel Ángel Rodriguez-Milla
    • Isabel Mirones
    • Luis Mariñas-Pardo
    • Gustavo J. Melen
    • Isabel Cubillo
    • Manuel Ramírez
    • Javier García-Castro
  • View Affiliations / Copyright

    Affiliations: Cellular Biotechnology Unit, Institute of Health Carlos III, Majadahonda, Madrid, Spain, Department of Oncohematology, Hospital Niño Jesús, Madrid, Spain
  • Pages: 365-373
    |
    Published online on: May 22, 2012
       https://doi.org/10.3892/ijmm.2012.1008
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Abstract

Neuroblastoma (NB) is one of the most common pediatric solid tumors and, like most human cancers, is characterized by a broad variety of genomic alterations. Although mesenchymal stem cells (MSCs) are known to interact with cancer cells, the relationship between MSCs and metastatic NB cancer cells in bone marrow (BM) is unknown. To obtain genetic evidence about this interaction, we isolated ΒΜ-derived MSCs from children with NB and compared their global expression patterns with MSCs obtained from normal pediatric donors, using the Agilent 44K microarrays. Significance analysis of microarray results with a false discovery rate (FDR) <5% identified 496 differentially expressed genes showing either a 2-fold upregulation or downregulation between both groups of samples. Comparison of gene ontology categories of differentially expressed genes revealed the upregulation of genes categorized as ‘neurological system process’, ‘cell adhesion’, ‘apoptosis’, ‘cell surface receptor linked signal transduction’, ‘intrinsic to membrane’ and ‘extracellular region’. Among the downregulated genes, several immunology-related terms were the most abundant. These findings provide preliminary genetic evidence of the interaction between MSCs and NB cancer cells in ΒΜ as well as identify relevant biological processes potentially altered in MSCs in response to NB.
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Copy and paste a formatted citation
Spandidos Publications style
Rodriguez-Milla MÁ, Mirones I, Mariñas-Pardo L, Melen GJ, Cubillo I, Ramírez M and García-Castro J: Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients. Int J Mol Med 30: 365-373, 2012.
APA
Rodriguez-Milla, M.Á., Mirones, I., Mariñas-Pardo, L., Melen, G.J., Cubillo, I., Ramírez, M., & García-Castro, J. (2012). Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients. International Journal of Molecular Medicine, 30, 365-373. https://doi.org/10.3892/ijmm.2012.1008
MLA
Rodriguez-Milla, M. Á., Mirones, I., Mariñas-Pardo, L., Melen, G. J., Cubillo, I., Ramírez, M., García-Castro, J."Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients". International Journal of Molecular Medicine 30.2 (2012): 365-373.
Chicago
Rodriguez-Milla, M. Á., Mirones, I., Mariñas-Pardo, L., Melen, G. J., Cubillo, I., Ramírez, M., García-Castro, J."Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients". International Journal of Molecular Medicine 30, no. 2 (2012): 365-373. https://doi.org/10.3892/ijmm.2012.1008
Copy and paste a formatted citation
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Spandidos Publications style
Rodriguez-Milla MÁ, Mirones I, Mariñas-Pardo L, Melen GJ, Cubillo I, Ramírez M and García-Castro J: Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients. Int J Mol Med 30: 365-373, 2012.
APA
Rodriguez-Milla, M.Á., Mirones, I., Mariñas-Pardo, L., Melen, G.J., Cubillo, I., Ramírez, M., & García-Castro, J. (2012). Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients. International Journal of Molecular Medicine, 30, 365-373. https://doi.org/10.3892/ijmm.2012.1008
MLA
Rodriguez-Milla, M. Á., Mirones, I., Mariñas-Pardo, L., Melen, G. J., Cubillo, I., Ramírez, M., García-Castro, J."Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients". International Journal of Molecular Medicine 30.2 (2012): 365-373.
Chicago
Rodriguez-Milla, M. Á., Mirones, I., Mariñas-Pardo, L., Melen, G. J., Cubillo, I., Ramírez, M., García-Castro, J."Enrichment of neural-related genes in human mesenchymal stem cells from neuroblastoma patients". International Journal of Molecular Medicine 30, no. 2 (2012): 365-373. https://doi.org/10.3892/ijmm.2012.1008
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