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Article

A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads

  • Authors:
    • Akikazu Sakudo
    • Kazuyoshi Ikuta
  • View Affiliations / Copyright

    Affiliations: Department of Virology, Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
  • Pages: 437-442
    |
    Published online on: May 22, 2012
       https://doi.org/10.3892/ijmm.2012.1009
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Abstract

Magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate) [poly(MVE-MA)], were used in a method to capture human immunodeficiency virus type-1 (HIV-1). The beads were incubated with either HIV-1-infected cell culture medium or plasma from HIV-1 infected individuals and separated from the supernatant by applying a magnetic field. After thorough washing, adsorption of HIV-1 by the beads was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR), real-time PCR, enzyme-linked immunosorbent assay and western blotting. The results confirmed the presence of envelope, polymerase, Nef and the viral genome of HIV-1. Furthermore, various subtypes and circulating recombinant forms (CRFs) of HIV-1 including subtype B, C and CRF01_AE and the immature form of subtype B HIV-1 could be captured. Preincubation with neutralizing antibody against HIV-1 envelope gp41 decreased the capture efficiently, suggesting that poly(MVE-MA) binds HIV-1 via gp41. We believe that this capture procedure will be a valuable tool for detecting various types of HIV-1 in both clinical and experimental samples.
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Copy and paste a formatted citation
Spandidos Publications style
Sakudo A and Ikuta K: A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads. Int J Mol Med 30: 437-442, 2012.
APA
Sakudo, A., & Ikuta, K. (2012). A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 30, 437-442. https://doi.org/10.3892/ijmm.2012.1009
MLA
Sakudo, A., Ikuta, K."A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 30.2 (2012): 437-442.
Chicago
Sakudo, A., Ikuta, K."A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 30, no. 2 (2012): 437-442. https://doi.org/10.3892/ijmm.2012.1009
Copy and paste a formatted citation
x
Spandidos Publications style
Sakudo A and Ikuta K: A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads. Int J Mol Med 30: 437-442, 2012.
APA
Sakudo, A., & Ikuta, K. (2012). A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 30, 437-442. https://doi.org/10.3892/ijmm.2012.1009
MLA
Sakudo, A., Ikuta, K."A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 30.2 (2012): 437-442.
Chicago
Sakudo, A., Ikuta, K."A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads". International Journal of Molecular Medicine 30, no. 2 (2012): 437-442. https://doi.org/10.3892/ijmm.2012.1009
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