Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
September 2012 Volume 30 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2012 Volume 30 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells

  • Authors:
    • Haiying Guo
    • Ke Yang
    • Fang Deng
    • Yizhan Xing
    • Yuhong Li
    • Xiaohua Lian
    • Tian Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cell Biology, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 636-642
    |
    Published online on: June 14, 2012
       https://doi.org/10.3892/ijmm.2012.1028
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Melanocytes are pigment-producing cells responsible for coloration of skin and hair. Although the importance of Wnt3a in melanocyte development has been well recognized, the role of Wnt3a in mature melanocytes has not been elucidated. This study was conducted to further explore the effects of Wnt3a on melanocyte proliferation and melanogenesis, and to elucidate the possible mechanisms involved. We infected melan-a cells with AdWnt3a to serve as the production source of the Wnt3a protein. MTT assay, 5-bromodeoxyuridine incorporation assay and flow cytometric analysis showed that Wnt3a inhibited the proliferation of melan-a cells and this was associated with decrease of cells in the S phase and increase of cells in the G1 phase. Melanin content and tyrosinase activity assay revealed that Wnt3a significantly promoted melanogenesis of melan-a cells. Furthermore, western blot analysis showed that Wnt3a upregulated the expression of microphthalmia-associated transcription factor and its downstream target genes, tyrosinase and tyrosinase-related protein 1 in melan-a cells. Collectively, our results suggest that Wnt3a plays an important role in melanocyte homeostasis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1. 

Y YamaguchiM BrennerVJ HearingThe regulation of skin pigmentationJ Biol Chem2822755727561200710.1074/jbc.R70002620017635904

2. 

A SlominskiJ WortsmanPM PlonkaKU SchallreuterR PausDJ TobinHair follicle pigmentationJ Invest Dermatol1241321200510.1111/j.0022-202X.2004.23528.x

3. 

A UongLI ZonMelanocytes in development and cancerJ Cell Physiol2223841201010.1002/jcp.2193519795394

4. 

RM WhiteLI ZonMelanocytes in development, regeneration, and cancerCell Stem Cell3242252200810.1016/j.stem.2008.08.00518786412

5. 

HY ParkM KosmadakiM YaarBA GilchrestCellular mechanisms regulating human melanogenesisCell Mol Life Sci6614931506200910.1007/s00018-009-8703-819153661

6. 

A SlominskiDJ TobinS ShibaharaJ WortsmanMelanin pigmentation in mammalian skin and its hormonal regulationPhysiol Rev8411551228200410.1152/physrev.00044.200315383650

7. 

J CuiLY ShenGC WangRole of hair follicles in the repigmentation of vitiligoJ Invest Dermatol97410416199110.1111/1523-1747.ep124809971714927

8. 

EK NishimuraSR GranterDE FisherMechanisms of hair graying: incomplete melanocyte stem cell maintenance in the nicheScience307720724200510.1126/science.109959315618488

9. 

T PasseronF MantouxJP OrtonneGenetic disorders of pigmentationClin Dermatol235667200510.1016/j.clindermatol.2004.09.013

10. 

JR MillerThe WntsGenome Biol3review30012002

11. 

DM EisenmannWnt signalingWormBook1172005(www.wormbook.org/toc_signaltrans.html)

12. 

JK SethiA Vidal-PuigWnt signalling and the control of cellular metabolismBiochem J427117201010.1042/BJ2009186620226003

13. 

RT MoonJD BrownM TorresWNTs modulate cell fate and behavior during vertebrate developmentTrends Genet13157162199710.1016/S0168-9525(97)01093-79097727

14. 

RI DorskyRT MoonDW RaibleControl of neural crest cell fate by the Wnt signalling pathwayNature396370373199810.1038/246209845073

15. 

RI DorskyRT MoonDW RaibleNeural crest-directed gene transfer demonstrates Wnt1 role in melanocyte expansion and differentiation during mouse developmentProc Natl Acad Sci USA971005010055200010.1073/pnas.97.18.1005010963668

16. 

KJ DunnM BradyCO JamborS SnyderA IncaoWJ PavanWNT1 and WNT3a promote expansion of melanocytes through distinct modes of actionPigment Cell Res18167180200510.1111/j.1600-0749.2005.00226.x15892713

17. 

EJ JinCA EricksonS TakadaLW BurrusWnt and BMP signaling govern lineage segregation of melanocytes in the avian embryoDev Biol2332237200110.1006/dbio.2001.022211319855

18. 

M IkeyaSM LeeJE JohnsonAP McMahonS TakadaWnt signalling required for expansion of neural crest and CNS progenitorsNature389966970199710.1038/401469353119

19. 

Y YamaguchiS ItamiH WatabeMesenchymal-epithelial interactions in the skin: increased expression of dickkopf1 by palmoplantar fibroblasts inhibits melanocyte growth and differentiationJ Cell Biol165275285200410.1083/jcb.200311122

20. 

Y YamaguchiT PasseronT HoashiDickkopf 1 (DKK1) regulates skin pigmentation and thickness by affecting Wnt/beta-catenin signaling in keratinocytesFASEB J2210091020200810.1096/fj.07-9475com17984176

21. 

DC BennettPJ CooperIR HartA line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growthInt J Cancer39414418198710.1002/ijc.29103903243102392

22. 

J LuoZL DengX LuoA protocol for rapid generation of recombinant adenoviruses using the AdEasy systemNat Protoc212361247200710.1038/nprot.2007.13517546019

23. 

Y PengQ KangH ChengTranscriptional characterization of bone morphogenetic proteins (BMPs)-mediated osteogenic signalingJ Cell Biochem9011491165200310.1002/jcb.1074414635189

24. 

EK NishimuraM SuzukiV IgrasKey roles for transforming growth factor beta in melanocyte stem cell maintenanceCell Stem Cell6130140201010.1016/j.stem.2009.12.01020144786

25. 

HR WidlundDE FisherMicrophthalamia-associated transcription factor: a critical regulator of pigment cell development and survivalOncogene2230353041200310.1038/sj.onc.120644312789278

26. 

E SteingrimssonNG CopelandNA JenkinsMelanocytes and the microphthalmia transcription factor networkAnnu Rev Genet38365411200410.1146/annurev.genet.38.072902.092717

27. 

S ShibaharaK YasumotoS AmaeRegulation of pigment cell-specific gene expression by MITFPigment Cell Res13Suppl 8S98S102200010.1034/j.1600-0749.13.s8.18.x

28. 

MA ChaconL Varela-NallarNC InestrosaFrizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomersJ Cell Physiol217215227200810.1002/jcp.2149718521822

29. 

H YamamotoH KomekadoA KikuchiCaveolin is necessary for Wnt-3a-dependent internalization of LRP6 and accumulation of beta-cateninDev Cell11213223200610.1016/j.devcel.2006.07.00316890161

30. 

H YamamotoH SakaneH YamamotoT MichiueA KikuchiWnt3a and Dkk1 regulate distinct internalization pathways of LRP6 to tune the activation of beta-catenin signalingDev Cell53748200810.1016/j.devcel.2008.04.01518606139

31. 

Y EndoE BeauchampD WoodsWnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3-and c-Jun N-terminal kinase-dependent mechanismMol Cell Biol2823682379200810.1128/MCB.01780-0718212053

32. 

H KomekadoH YamamotoT ChibaA KikuchiGlycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3aGenes Cells12521534200710.1111/j.1365-2443.2007.01068.x17397399

33. 

P RabbaniM TakeoW ChouCoordinated activation of wnt in epithelial and melanocyte stem cells initiates pigmented hair regenerationCell145941955201110.1016/j.cell.2011.05.00421663796

34. 

YC ShangSH WangF XiongWnt3a signaling promotes proliferation, myogenic differentiation, and migration of rat bone marrow mesenchymal stem cellsActa Pharmacol Sin2817611774200710.1111/j.1745-7254.2007.00671.x17959027

35. 

L JiaJ ZhouS PengJ LiY CaoE DuanEffects of Wnt3a on proliferation and differentiation of human epidermal stem cellsBiochem Biophys Res Commun368483488200810.1016/j.bbrc.2008.01.09718242164

36. 

GM BolandG PerkinsDJ HallRS TuanWnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cellsJ Cell Biochem9312101230200410.1002/jcb.2028415486964

37. 

MK NygrenG DosenME HystadH StubberudS FunderudE RianWnt3A activates canonical Wnt signalling in acute lymphoblastic leukaemia (ALL) cells and inhibits the proliferation of B-ALL cell linesBr J Haematol136400413200710.1111/j.1365-2141.2006.06442.x17156404

38. 

V DelmasF BeermannS MartinozziBeta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma developmentGenes Dev2129232935200710.1101/gad.45010718006687

39. 

M ChoM RyuY JeongCardamonin suppresses melanogenesis by inhibition of Wnt/beta-catenin signalingBiochem Biophys Res Commun390500505200910.1016/j.bbrc.2009.09.12419800318

40. 

I AksanCR GodingTargeting the microphthalmia basic helix-loop-helix-leucine zipper transcription factor to a subset of E-box elements in vitro and in vivoMol Cell Biol186930693819989819381

41. 

K TakedaK YasumotoR TakadaInduction of melanocyte-specific microphthalmia-associated transcription factor by Wnt-3aJ Biol Chem2751401314016200010.1074/jbc.C00011320010747853

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo H, Yang K, Deng F, Xing Y, Li Y, Lian X and Yang T: Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Int J Mol Med 30: 636-642, 2012.
APA
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., & Yang, T. (2012). Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. International Journal of Molecular Medicine, 30, 636-642. https://doi.org/10.3892/ijmm.2012.1028
MLA
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., Yang, T."Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells". International Journal of Molecular Medicine 30.3 (2012): 636-642.
Chicago
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., Yang, T."Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells". International Journal of Molecular Medicine 30, no. 3 (2012): 636-642. https://doi.org/10.3892/ijmm.2012.1028
Copy and paste a formatted citation
x
Spandidos Publications style
Guo H, Yang K, Deng F, Xing Y, Li Y, Lian X and Yang T: Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Int J Mol Med 30: 636-642, 2012.
APA
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., & Yang, T. (2012). Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. International Journal of Molecular Medicine, 30, 636-642. https://doi.org/10.3892/ijmm.2012.1028
MLA
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., Yang, T."Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells". International Journal of Molecular Medicine 30.3 (2012): 636-642.
Chicago
Guo, H., Yang, K., Deng, F., Xing, Y., Li, Y., Lian, X., Yang, T."Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells". International Journal of Molecular Medicine 30, no. 3 (2012): 636-642. https://doi.org/10.3892/ijmm.2012.1028
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team