Interleukin-13 peptide kinoid vaccination attenuates allergic inflammation in a mouse model of asthma

Jing,Qiuping; Yin,Tao; Wan,Yang; Shi,Huashan; Luo,Shuntao; Li,Meng; Zhang,Hailong; He,Hong; Liu,Shijia; Li,Haoyu; Wei,Yuquan; Yang,Li

doi:10.3892/ijmm.2012.1036

Interleukin-13 peptide kinoid vaccination attenuates allergic inflammation in a mouse model of asthma

Authors:
- Qiuping Jing
- Tao Yin
- Yang Wan
- Huashan Shi
- Shuntao Luo
- Meng Li
- Hailong Zhang
- Hong He
- Shijia Liu
- Haoyu Li
- Yuquan Wei
- Li Yang
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Affiliations: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, P.R. China, School of Life Science, Sichuan University, Chengdu, Sichuan, P.R. China
Published online on: June 19, 2012 https://doi.org/10.3892/ijmm.2012.1036
Pages: 553-560

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Abstract

Asthma is an atopic disorder with increasing frequency and severity in developed nations. Interleukin-13 (IL-13) is one of the most critical mediators of asthma pathology. In the present study, we developed a vaccine comprised of a keyhole limpet hemocyanin-mIL-13 heterocomplex immunogen to persistently neutralize excessive endogenous IL-13. Our results showed that the IL-13 peptide kinoid vaccine could induce sustained and high titer of IL-13-specific IgG when using aluminum hydroxide as an adjuvant, and could suppress the accumulation of eosinophils as well as IL-13 levels in bronchoalveolar lavage fluid (BALF). In addition, total IgE and ovalbumin (OVA)-specific IgE in serum were significantly inhibited. This study also showed that vaccination could prevent airway inflammation and epithelial cell proliferation with goblet cell hyperplasia in a mouse model of acute asthma. In summary, our findings suggest that the IL-13 peptide kinoid can serve as an innovative and effective vaccine against asthma.

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