Osteogenic differentiation of human periodontal ligament stem cells expressing lentiviral NEL-like protein 1

Chen,Cai-Yun; Liu,Ya-Jing; Shi,Sheng-Gen; Chen,Fa-Ming; Cai,Chuan; Li,Bing; Wang,Jing; Shi,Liang; Li,Yan; Liu,Zhong-Yu; Niu,Zhong-Ying

doi:10.3892/ijmm.2012.1053

October 2012 Volume 30 Issue 4

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October 2012 Volume 30 Issue 4

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Article

Osteogenic differentiation of human periodontal ligament stem cells expressing lentiviral NEL-like protein 1

Authors:
- Cai-Yun Chen
- Ya-Jing Liu
- Sheng-Gen Shi
- Fa-Ming Chen
- Chuan Cai
- Bing Li
- Jing Wang
- Liang Shi
- Yan Li
- Zhong-Yu Liu
- Zhong-Ying Niu
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Affiliations: Chinese PLA Postgraduate Medical School, Beijing, P.R. China, Institute of Stomatology, Chinese PLA General Hospital, Beijing, P.R. China, Center of Stomatology, The 306th Hospital of PLA, Beijing, P.R. China, Center Laboratory for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shanxi, P.R. China, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shanxi, P.R. China, Department of Gynecology, Chinese PLA General Hospital, Beijing, P.R. China
Pages: 863-869
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Published online on: July 4, 2012

https://doi.org/10.3892/ijmm.2012.1053
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Abstract

NEL-like protein 1 (NELL1) is a newly identified secreted protein involved in craniosynostosis and has been found to promote osteogenic differentiation of mesenchymal stem cells. The objective of this study was to investigate the effect of NELL1 on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and the potential underlying mechanism. hPDLSCs underwent lentivirus-mediated NELL1 transfection (Lenti-NELL1) and markers of osteogenesis were assessed [alkaline phosphate (ALP), osteocalcin (OCN) and calcium deposition] to evaluate the effect of NELL1 on the differentiation of these cells. Quantitative polymerase chain reaction (qPCR) was employed to measure the mRNA expression of Msx2 and Runx2, and Lenti-enhanced green fluorescent protein (EGFP) served as a control. Western blot analysis and qPCR analyses confirmed that Lenti-NELL1-transfected hPDLSCs could express NELL1. Compared with the Lenti-EGFP group, ALP, OCN, calcium deposition and Msx2 mRNA expression were markedly increased (P<0.01), but there was no significant difference in Runx2 mRNA expression between the two groups (P>0.01). hPDLSCs can be transfected by Lenti-NELL1 and can stably express NELL1. NELL1 is able to promote the osteogenic differentiation of hPDLSCs, which may be related to the downregulation of Msx2 expression. Lenti-NELL1 transfection can be used during in vitro gene therapy for periodontal regeneration.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Osteogenic differentiation of human periodontal ligament stem cells expressing lentiviral NEL-like protein 1

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