Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells

  • Authors:
    • Eduardo Parra
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  • Published online on: July 5, 2012     https://doi.org/10.3892/ijmm.2012.1055
  • Pages: 923-930
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Abstract

Previous studies have shown that c-Jun-N-terminal kinase-1 (JNK-1) is involved in the transformation of primary fibroblasts and plays a role in tumor cell growth. A number of observations suggest that JNK-1 is a growth promoting factor in prostate cancer cells and blocking its function may induce apoptosis. To test this further, we used a small interfering RNA (siRNA) against JNK-1 mRNA that efficiently inhibits JNK-1 expression in the prostate cancer cell line, PC-3. The application of siRNA against JNK-1 decreased the expression of JNK-1 and affected the expression of p21, XIAP and Bcl-2, but had no effect on the expression of VEGF. In contrast, a control scramble siRNA did not affect the expression of the above indicated proteins. The downregulation of JNK-1 expression at both the mRNA and protein levels was detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. Cell proliferation inhibition rates were determined by the MTT assay. The effect of JNK-1-siRNA on cell cycle distribution and cell apoptosis was determined by flow cytometry, DNA fragmentation and caspase activity. Our data showed that siRNA against JNK-1 mRNA, could efficiently suppress the expression of JNK-1 in PC-3 cells. After 5 days of transfection, the cell death rate was 52%, the apoptotic rate 26% and the viability rate 22%. In conclusion, downregulation of JNK-1 expression by siRNA against JNK-1 mRNA induces apoptotic signaling in prostate cancer PC-3 cells. The use of siRNA against JNK-1 as a novel approach to cancer therapy deserves further investigation.
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October 2012
Volume 30 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Parra E: Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells. Int J Mol Med 30: 923-930, 2012
APA
Parra, E. (2012). Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells. International Journal of Molecular Medicine, 30, 923-930. https://doi.org/10.3892/ijmm.2012.1055
MLA
Parra, E."Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells". International Journal of Molecular Medicine 30.4 (2012): 923-930.
Chicago
Parra, E."Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells". International Journal of Molecular Medicine 30, no. 4 (2012): 923-930. https://doi.org/10.3892/ijmm.2012.1055